Experimental method for using paeonol as aspergillus flavus bacteriostatic agent
An experimental method and technology of Aspergillus flavus, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of high probability of carcinogenicity, strong toxicity, and threat to human health, and achieve the effect of convenient operation and high reliability.
Active Publication Date: 2021-01-29
HENAN UNIVERSITY OF TECHNOLOGY
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At the same time, Aspergillus flavus produces aflatoxin, which is highly toxic and has a high chance of causing cancer, which seriously threatens human health.
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Embodiment 1
[0039] A kind of experimental method that paeonol is used as aspergillus flavus antibacterial agent comprises the following steps,
[0040] Step 1, prepare medium, prepare two groups of medium groups, respectively the first medium group and the second medium group, the first medium group is SDA (Sabouraud dextrose agar medium), the second medium group is SDB (Sabouraud Dextrose Broth);
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Abstract
The invention relates to paeonol, in particular to an experimental method for using paeonol as an aspergillus flavus bacteriostatic agent, which introduces paeonol as an aspergillus flavus inhibitor,has the advantages of high efficiency, no toxicity, environmental friendliness, economical efficiency, applicability and the like, and can quickly judge whether the paeonol can inhibit aspergillus flavus or not by detecting the influence of paeonol with different concentrations on aspergillus flavus hyphae. The bacteriostatic concentration low value of paeonol can be obtained. The method is convenient to operate, the bacteriostatic mechanism of the paeonol can be accurately judged through mycelium collection and mechanism determination, the inhibitory effect of the paeonol on the aspergillus flavus can be verified, and a basis can be provided for selection of application occasions. Through surface ultrastructure detection, internal detection, diaphragm number detection, chitin content detection and beta-1, 3-glucan detection, the antibacterial mechanism can be comprehensively judged, verification is implemented through a verification test, the effectiveness of the paeonol as the aspergillus flavus bacteriostatic agent can be verified, a complete experimental system is formed, and the reliability is high.
Description
technical field [0001] The invention relates to paeonol, in particular to paeonol used as an antibacterial agent for Aspergillus flavus. Background technique [0002] Paeonol is an active ingredient of traditional Chinese medicine extracted from the root bark of Moutan. This drug has anti-inflammatory, labor pain, antipyretic and anti-allergic effects. At the same time, paeonol has broad-spectrum antibacterial properties, and has inhibitory effects on both bacteria and fungi. However, there are few reports on paeonol's inhibition of Aspergillus flavus, and the antibacterial mechanism has not yet been proposed. [0003] Aspergillus flavus, a common fungus, is extremely prone to contamination during postharvest storage and food processing. As an opportunistic pathogen, it can also cause aspergillosis in humans and animals. At the same time, Aspergillus flavus produces aflatoxin, which is highly toxic and has a high chance of causing cancer, which seriously threatens human hea...
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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 李倩朱晓嫚赵颖
Owner HENAN UNIVERSITY OF TECHNOLOGY
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