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Microgel as surface-enhanced Raman scattering substrate and its preparation method and use

A surface-enhanced Raman, microgel technology, applied in Raman scattering, nanotechnology for materials and surface science, transportation and packaging, etc. , to achieve the effect of a simple preparation method

Active Publication Date: 2022-03-29
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application aims at the problem that the preparation method of microgel is relatively cumbersome, requires the regulation of precision equipment, and is not suitable for widespread promotion, and provides a microgel as a surface-enhanced Raman scattering substrate

Method used

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  • Microgel as surface-enhanced Raman scattering substrate and its preparation method and use
  • Microgel as surface-enhanced Raman scattering substrate and its preparation method and use
  • Microgel as surface-enhanced Raman scattering substrate and its preparation method and use

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Experimental program
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Effect test

Embodiment 1

[0037] The preparation method of gold nano double cone solution:

[0038] Refer to the article published by Professor Luis M. Liz-Marzán's research group on JACS in 2017 to synthesize gold nanobiconic nanoparticles. The synthesis process is divided into two parts:

[0039] (1) Synthesis of gold species: 0.25mL 25mM freshly prepared frozen sodium borohydride solution was added to 10mL mixed solution (0.25mM chloroauric acid, 50mM CTAC, 5.0mM citric acid) and reacted at 80°C for 90min.

[0040] (2) Synthesis of gold nanobiconic nanoparticles: Add the above 8.0mL gold seed solution to 200 mL 100mMCTAB, 10mL, 10mM chloroauric acid, 2.0mL, 10mM silver nitrate, 4.0mL, 1.0 M hydrochloric acid, 1.6mL, In a mixed solution of 100 mM ascorbic acid, react for 2 hours.

Embodiment 2

[0042] Preparation of microgels as surface-enhanced Raman scattering substrates:

[0043] 0.50mL 1.0x10 -8 mol / L gold nano biconical solution was added to 10mL 0.1mol / L cetyltrimethylammonium bromide; then 0.50mL 10mg / mL polyethyleneimine and 0.50mL 10 mg / mL silver nitrate were added in turn; mix well Afterwards, react at 120° C. for 4 hours; separate the precipitate, and dissolve the precipitate with 1.0 mL of ultrapure water.

[0044] Add 100μL 5.0mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 100μL 1.0mg / mL N-hydroxysuccinimide to 200μL 1.0mg / mL mL of sodium hyaluronate solution, mixed evenly, reacted with slight shaking at room temperature for 15 minutes, then added 200 μL of precipitate solution, and reacted with slight shaking at room temperature for 6 hours to obtain a microgel.

[0045] The microgels of the present application as surface-enhanced Raman scattering substrates were characterized by high-resolution transmission electron microscopy (ST...

Embodiment 3

[0047] Microgel detection of rhodamine 6G as a surface-enhanced Raman scattering substrate:

[0048] Will contain 60μL rhodamine 6G (1.0x10 -6 M) Added to 50 μL microgel, shaken slightly for 30 minutes, and detected with a portable Raman instrument.

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Abstract

The application discloses a microgel as a surface-enhanced Raman scattering substrate and its preparation method and application. The microgel is in the form of particles, and its particles include gold nano-bicones in the inner layer, coated on the surface of the gold nano-bicones The silver nanometer and the outer hyaluronic acid hydrogel. The preparation method comprises the following steps: providing a gold nano bicone particle solution, mixing with a reducing agent and a silver ion solution for reduction reaction, separating and obtaining a precipitate; redissolving the precipitate in water, and mixing with sodium hyaluronate for condensation reaction to produce the microgel. The microgel is directly mixed with the sample to be tested, and the surface-enhanced Raman scattering detection is performed without pretreatment of the sample. The preparation method of the microgel of the present application is simple, easy to operate, and suitable for large-scale promotion.

Description

technical field [0001] The present application relates to the field of surface-enhanced Raman scattering detection, in particular to a microgel as a surface-enhanced Raman scattering substrate and its preparation method and application. Background technique [0002] Surface-enhanced Raman scattering (SERS) is a high-sensitivity in situ analysis technique, and when some molecules are adsorbed onto rough metal (Au, Ag, Cu, etc.) surfaces, their Raman scattering intensity increases significantly. [0003] Nanoparticles such as AuNPs have been used as conventional SERS substrates, but the poor reproducibility of detection results due to the poor dispersion of nanoparticles (NPs) in complex samples limits their wide application. To solve this problem, Professor Shin-Hyun Kim's research group used capillary microfluidic technology to prepare micron-scale gels, which can be used for the analysis and detection of complex mixtures. Professor Luis M. Liz-Marzán’s research group used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65B22F1/102B22F1/17B82Y40/00B82Y30/00
CPCG01N21/658B82Y40/00B82Y30/00B22F1/102B22F1/17
Inventor 郭隆华林丙永姚媛媛
Owner JIAXING UNIV