Immunochromatographic test strip for quantitatively detecting procalcitonin and quantitative detection method thereof

An immunochromatographic test strip, procalcitonin technology, applied in biological testing, measuring devices, analytical materials, etc., can solve problems such as low detection sensitivity, and achieve the effects of simple operation, improved linear range and stability, and rapid detection.

Inactive Publication Date: 2021-02-02
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Immunochromatographic test strip for quantitatively detecting procalcitonin and quantitative detection method thereof
  • Immunochromatographic test strip for quantitatively detecting procalcitonin and quantitative detection method thereof
  • Immunochromatographic test strip for quantitatively detecting procalcitonin and quantitative detection method thereof

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Embodiment 1

[0050] The optimization of embodiment 1 PCT labeling antibody optimum loading amount

[0051]In order to determine the optimal labeling amount of nano-gold-labeled PCT antibody, we carried out spectrophotometric determination on the gold-labeled antibody solution. The results of spectrophotometry were as figure 2 As shown in the figure, the absorbance values ​​measured at 525nm by adding 8, 10, 12, 14, 16, and 18 μL of 1 mg / mL PCT antibody solution respectively, it can be seen from the figure that when the amount of PCT antibody added reaches 12 μL and above , the absorbance value of the nano-gold mixture remains almost unchanged, so it is determined that the optimal antibody labeling amount of stable nano-gold is 12 μg / 1mL nano-gold, and an additional 20% is added on this basis, that is, 12×(1+0.2)=14.4 μg, for the convenience of adding, the final determination is to use 15 μg of PCT antibody addition / 1mL nano-gold.

Embodiment 2

[0052] Embodiment 2 detects procalcitonin with the test strip that the present invention makes

[0053] A kind of immunochromatographic test strip for quantitative detection of procalcitonin was used to detect different concentrations of procalcitonin standard samples (take 7 different concentrations, respectively 0, 0.1, 0.5, 2, 8, 14, 20ng / mL, three parallel samples were made for each concentration). After a period of chromatographic reaction, use a self-assembled fluorescent immunochromatographic analysis device to detect the fluorescence signals at the detection line T1 and control line T2, and obtain the relative fluorescence signal value T1 / T2, with the logarithm value of the PCT antigen concentration as the abscissa log C , with T1 / T2 as the ordinate y, establish an equation and fit it into a standard curve.

[0054] Depend on image 3 It can be seen from the standard curve that the T1 / T2 signal value and the logarithmic value of the PCT antigen concentration present...

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Abstract

The invention discloses an immunochromatographic test strip for quantitatively detecting procalcitonin and a quantitative detection method thereof. The test strip comprises a PVC lining plate, a nitrocellulose membrane (NC membrane), a combination pad, a sample pad and a water absorption pad; the PVC lining plate is sequentially overlapped and pasted with the sample pad, the combination pad, the NC membrane and the water absorption pad from left to right; the binding pad contains a nanogold labeled first antibody for resisting procalcitonin; the nitrocellulose membrane is provided with a detection area and a control area, fluorescent strips are respectively arranged in the detection area and the control area, a detection line is arranged on the fluorescent strip of the detection area, an anti-procalcitonin coated antibody is used as the detection line, a control line is arranged on the fluorescent strip of the control area, and a procalcitonin antigen is coated on the control line. Compared with a common method for detecting procalcitonin at present, the method has the advantages of simplicity and convenience in operation, quickness, convenience in carrying, small sample consumption and the like, and meanwhile, the linear range and the stability of detection are also improved.

Description

technical field [0001] The invention relates to procalcitonin detection technology, belongs to the field of immunochemical detection, in particular to an immunochromatographic test strip for quantitative detection of procalcitonin and a quantitative detection method thereof. Background technique [0002] Procalcitonin (PCT) is a glycoprotein synthesized in thyroid C cells. It is one of the biomarkers that reflect the systemic inflammation of the organism. It is a pro-peptide substance of calcitonin without calcitonin hormone activity. , The structure of PCT is composed of 116 amino acids. When the tissues and organs in the organism face the invasion of bacteria, fungi and other endotoxins and inflammatory factors, almost all PCT can be synthesized. [0003] When a bacterial infection occurs in the human body, the content of PCT in plasma can rise sharply in a short period of time and maintain a high level within 24 hours. When diseases such as aseptic inflammation and viral ...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/58G01N33/558
CPCG01N33/74G01N33/582G01N33/558G01N2333/585G01N2800/7095
Inventor 潘宏程王雅茹
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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