A kind of preparation method of meso-2,3-butanediol
A technology of butanediol and meso, which is applied in the field of preparation of meso-2,3-butanediol, can solve the problems of skin toxicity, skin irritation, allergy and low skin irritation
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[0033] Based on the above findings, the present invention provides a method for preparing meso-2,3-butanediol, the preparation method comprising the following steps:
[0034] (1) Using renewable biomass as raw material, Klebsiella is used to ferment 2,3-butanediol to obtain 2,3-butanediol fermentation broth;
[0035] (2) The 2,3-butanediol fermentation broth obtained in step (1) is sequentially subjected to ultrafiltration for sterilization and protein removal, nanofiltration for protein and partial salt removal, electrodialysis for desalination, and Multi-effect evaporation to remove part of the water to obtain a concentrated solution;
[0036] (3) dehydrating the concentrated solution obtained in step (2) using the first rectification tower to obtain a dehydration liquid, and then distilling the dehydration liquid to obtain a distillate;
[0037] (4) The distillate obtained in step (3) is rectified in the second rectification tower, and 3-hydroxy-2-butanone is separated fro...
Embodiment 1
[0057] This example provides a preparation method of meso-2,3-butanediol, the preparation method comprising the following steps:
[0058] (1) Using renewable biomass (specifically glycerol) as raw material, Klebsiella is used to ferment 2,3-butanediol to obtain 2,3-butanediol fermentation liquid; the specific implementation method is as follows:
[0059] After inoculation in the fermenter, control the temperature of the fermentation broth to 37°C, pH value to 6.5, ventilation rate to 0.08vvm, stirring rate to 50rpm, measure the concentration of substrate glycerol in the fermentation broth during the fermentation process, and add glycerol according to the consumption rate of glycerol to ensure that the glycerol in the fermentation broth The concentration is 0.5-30g / L, fermented for 48 hours and put into the tank;
[0060] (2) The 2,3-butanediol fermentation liquid obtained in step (1) is sequentially carried out:
[0061] It is used for ultrafiltration for sterilization and pr...
Embodiment 2
[0077] This example provides a preparation method of meso-2,3-butanediol, the preparation method comprising the following steps:
[0078] (1) Using renewable biomass (specifically glycerol) as raw material, Klebsiella is used to ferment 2,3-butanediol to obtain 2,3-butanediol fermentation liquid; the specific implementation method is as follows:
[0079] After inoculation in the fermenter, control the temperature of the fermentation broth to 37°C, pH value to 6.5, ventilation rate to 0.08vvm, stirring rate to 50rpm, measure the concentration of substrate glycerol in the fermentation broth during the fermentation process, and add glycerol according to the consumption rate of glycerol to ensure that the glycerol in the fermentation broth The concentration is 0.5-30g / L, fermented for 48 hours and put into the tank;
[0080] (2) The 2,3-butanediol fermentation liquid obtained in step (1) is sequentially carried out:
[0081] It is used for ultrafiltration for sterilization and pr...
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