Stable coronavirus recombinant protein dimer and its expression vector

A coronavirus and recombinant protein technology, applied in the field of recombinant protein, can solve the problems of poor protein stability and weak immunogenicity, achieve good immunogenicity, avoid degradation, and improve immunogenicity
CN112321688BActive Publication Date: 2021-03-26SOUTH CHINA UNITED VACCINE INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA UNITED VACCINE INST
Publication Date
2021-03-26

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Abstract

The invention discloses a stable coronavirus recombinant protein dimer and its expression vector. The coronavirus recombinant protein consists of the coronavirus S protein S-RBD, the CTD region N-CTD of the coronavirus N protein and the connection connecting the two Sub composition. The coronavirus recombinant protein of some examples of the present invention can form and maintain a stable dimer structure, avoid the degradation of the monomer S-RBD, help improve the immunogenicity of the coronavirus recombinant protein, and is expected to be used for the preparation of raw materials for detection reagents and vaccines , antibodies, prophylactic or therapeutic drugs. The coronavirus recombinant protein dimers of some examples of the present invention have good immunogenicity. It has broad application prospects in the field of vaccine development. The expression vectors of some examples of the present invention are easy to express coronavirus recombinant protein dimers and have high expression levels.
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Description

technical field

[0001] The invention relates to a recombinant protein, in particular to a stable coronavirus recombinant protein dimer and its expression vector. Background technique

[0002] Coronaviruses belong to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. They are important pathogens of many domestic animals, pets, and human diseases, causing a variety of acute and chronic diseases. According to the phylogenetic tree, coronaviruses can be divided into four genera: α, β, γ, and δ, among which β coronaviruses can be divided into four independent subgroups A, B, C and D.

[0003] Coronavirus infections are extremely common around the world. So far, about 15 different coronavirus strains have been discovered, which can infect a variety of mammals and birds, and some can cause disease in humans. In addition to the recent outbreak of the new coronavirus SARS-CoV-2, there are six coronaviruses that can infect humans, including HCoV-229E, HCoV-O...

Claims

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