Extraction method of red date peel pigment

An extraction method, the technology of red jujube skin, applied in the field of extraction of red jujube skin pigment, can solve the problems such as compound enzyme combined with ultrasonic degradation of cell wall treatment method that has not been studied, and achieve good coloring effect, promote release, and simple process flow

Inactive Publication Date: 2021-02-05
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many researches on enzymes and ultrasound used to degrade cell walls, but there is no research on the treatment of compound enzymes combined with ultrasound to degrade cell walls

Method used

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  • Extraction method of red date peel pigment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Dry 500 grams of jujube dregs at a temperature of 45°C to obtain dark red jujube skins, and then crush them through an 80-mesh sieve; 25min), then sonicate (450W) for 5min, then add pectinase (1500000U) for enzymolysis (pH=6, temperature 40°C, time 25min), then sonicate (450W) for 5min, finally add protease (1000000U) for enzymolysis (pH =7, temperature 40°C, time 25min), then ultrasonic (450W) for 5min, after the enzymolysis is completed, inactivate the enzyme in a 90°C water bath for 5min. After inactivating the enzyme, add 0.04M NaOH (according to the ratio of solid to liquid: 1:25) for full extraction. The extract was adjusted to pH 7 with HCl, filtered by vacuum filtration, and the obtained filtrate was concentrated under reduced pressure at 45°C, and when the remaining liquid was only 15% of the original liquid, the concentration was stopped, allowed to cool to room temperature, and then removed. Vacuum dry in a vacuum freeze dryer to obtain 82 g of red pigment s...

Embodiment 2

[0015] Dry 50 grams of jujube dregs at a temperature of 40°C to obtain dark red jujube skins, and then crush them through an 80-mesh sieve; add cellulase (200000U) to enzymatically hydrolyze the obtained jujube skins (pH=5.5, temperature 40°C, time 25min), then sonicate (500W) for 5min, then add pectinase (200000U) for enzymolysis (pH=6.5, temperature 40°C, time 25min), then sonicate (500W) for 5min, finally add protease (100000U) for enzymolysis (pH =7.5, temperature 40°C, time 25min), then ultrasonic (500W) for 5min, after the enzymolysis is completed, inactivate the enzyme in a 90°C water bath for 5min. Add 0.02M NaOH (according to the ratio of solid to liquid 1:25) to fully extract after the enzyme inactivation is completed. The pH value of the extract was adjusted to 7 with HCl, filtered by vacuum filtration, and the obtained filtrate was concentrated under reduced pressure at 50°C, and when the remaining liquid was only 20% of the original liquid, the concentration was s...

Embodiment 3

[0017] Dry 5 grams of jujube dregs at a temperature of 50°C to obtain dark red jujube skins, and then crush them through an 80-mesh sieve; 25min), then sonicate (600W) for 5min, then add pectinase (15000U) for enzymolysis (pH=5.5, temperature 40°C, time 25min), then sonicate (600W) for 5min, finally add protease (9000U) for enzymolysis (pH =6.5, temperature 40°C, time 25min), then ultrasonic (600W) for 5min, after enzymolysis, inactivate the enzyme in a 90°C water bath for 5min. Add 0.05M NaOH (according to the ratio of solid to liquid: 1:25) to fully extract after the enzyme inactivation is completed. The extract was adjusted to pH 7 with HCl, filtered by vacuum filtration, and the resulting filtrate was concentrated under reduced pressure at 50°C. When the remaining liquid was only 10% of the original liquid, the concentration was stopped, and allowed to cool to room temperature, and then removed. Vacuum-dry in a vacuum freeze dryer to obtain 0.68 g of a red pigment solid. ...

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Abstract

The invention relates to an extraction method of the red date peel pigment, which comprises the following steps: drying and crushing red date residues; using cellulase for assisting in enzymolysis ofthe red date peel, using pectinase for assisting in enzymolysis of the red date peel, and finally using protease for assisting in enzymolysis of the red date peel; performing enzyme deactivation afterenzymolysis and ultrasonic treatment are completed; extracting by using NaOH, and taking a supernatant; adjusting the pH value of the supernatant, filtering by adopting a vacuum filtration method, and concentrating the obtained filtrate under reduced pressure; and transferring the concentrated solution to a vacuum freeze dryer for vacuum freeze drying to obtain the red date peel pigment. The process flow is simple, the cost is low, the extracted pigment is natural pigment, the color is natural, and the pharmacological functions of tonifying spleen and stomach, tonifying qi and nourishing blood and the like are achieved.

Description

technical field [0001] The invention belongs to the field of fine chemicals, and in particular relates to a method for extracting a pigment, in particular to a method for extracting a pigment from jujube skin. Background technique [0002] At present, the research on pigments has become an indispensable part of the modern food industry. Most of them are artificially synthesized. Synthetic pigment additives are not the original ingredients of food, and they are ingested together with food, which is easy to induce poisoning, diarrhea and even cancer. It is harmful to the human body, so it cannot be used more or should not be used as much as possible. However, natural pigments are safe and non-toxic coloring agents. The study of natural pigments allows people to further enter the fast era of modern industrial development, promotes the modernization of the food industry, and at the same time increases the speed of China's light industry development and is at the forefront of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54A23L5/43A23L33/105
CPCA23V2002/00A23L5/43A23L33/105C09B61/00C09B67/0096A23V2200/04A23V2200/30A23V2200/32A23V2250/21A23V2250/2116A23V2300/48
Inventor 吴彩娥沈东北窦金凤范龚健李婷婷
Owner NANJING FORESTRY UNIV
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