Immunoassay device for in vitro immediate determination of human ABO and/or Rh blood types

Abstract

The invention discloses an immunoassay device for in-vitro immediate determination of human ABO and / or Rh blood types. The immunoassay device comprises an upper cover, reaction films, absorption liners, a back liner and a base. A plurality of sample holes are formed in the upper cover, the reaction film is arranged below each sample hole, the reaction films are respectively coated with antibodiesfor measuring blood types or positive quality control internal standard substances and negative quality control internal standard substances for monitoring reaction effectiveness, and the absorption liners and the reaction films are arranged side by side at intervals, so that the reaction films below the adjacent holes are separated; the back liner is located on the opposite side of the upper cover relative to the reaction films and the absorption liners, one surface of the back liner is attached to the reaction films and the absorption liners, and the other surface of the back liner is in contact with the base and supported and fixed by the base. The immunoassay device for in-vitro immediate determination of the human body ABO and / or Rh blood types is provided with a quality control internal standard, can be used for various situations such as hospitals, families and field emergencies, and can rapidly, accurately, simply and conveniently detect the ABO and / or Rh blood types of the human body.

Description

technical field [0001] The invention relates to the field of medical blood type detection, in particular to an immunoassay device for instantly measuring human ABO and / or Rh blood types in vitro. Background technique [0002] Human blood typing techniques are divided into two categories, genotyping by detecting DNA and serological typing by detecting antigen / antibody. Most of the blood typing detection methods currently used in clinical testing are only suitable for use in central laboratories. The problems of slow detection speed, inconvenient operation and high cost cannot be avoided. In recent years, with the sinking of medical resources and the implementation of hierarchical diagnosis and treatment, there is an urgent need for a simple, fast and accurate blood typing detection technology. [0003] In this regard, many people have conducted some explorations and proposed some technical solutions, but these technical solutions generally have the following problems: fast ...

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Problems solved by technology

[0003] In this regard, many people have conducted some explorations and proposed some technical solutions, but these technical solutions generally have the following problems: fast and inaccurate, lack of quality control, inconvenient operation, difficult to manufacture, high cost

However, the antigen and antibody at the quality control line need a second reaction, which increases the detection time

At the same time, because the color of the blood is red, the test line and quality control line are interpreted according to the red color, the error is large, and the specificity needs to be improved.

[0006] The Chinese patent application with the application number 201811096877.X discloses a reagent card for detecting human ABO and RhD blood typing using the percolation method, but lacks quality control and is prone to false positives and false negatives

The process needs to be left to stand for a period of time, and immediate detection is not possible

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