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Major QTL for regulating and controlling resistance of sogatella furcifera of rice, molecular marker and application

A white-backed planthopper and molecular marker technology, applied in the field of rice breeding and molecular biology, can solve the problems of fine positioning and limited research on related molecular markers, and achieve the effect of speeding up the selection and breeding process

Active Publication Date: 2021-02-19
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, researchers have limited research on the fine mapping of rice QTL loci for resistance to white-backed planthopper and related molecular markers; therefore, it is necessary to further dig and analyze the QTL loci and related molecular markers for rice resistance to white-backed planthopper. Screening and identification of rice resistant varieties provide new options

Method used

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  • Major QTL for regulating and controlling resistance of sogatella furcifera of rice, molecular marker and application
  • Major QTL for regulating and controlling resistance of sogatella furcifera of rice, molecular marker and application
  • Major QTL for regulating and controlling resistance of sogatella furcifera of rice, molecular marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Mapping of major QTLs regulating rice white-backed planthopper resistance

[0034] 1. Acquisition of experimental materials

[0035] Reyan 2 was used as the donor parent, and the rice variety Huazhan was used as the recipient parent for crossing, and the single-seed method was used (that is, F1 was treated with bagging and planting a single plant until the phenotype of the offspring lines did not segregate). , and finally got 120 stable genetic lines (F13, all lines are phenotypically stable), forming a recombinant inbred line RIL population, such as figure 1 .

[0036] Select 60 seeds (F13) of the parent and each strain, soak the seeds for 2 days after surface disinfection, wrap them in a moist towel, put them in a 37°C incubator for 48 hours, and select the seeds with consistent dew and whiteness for sowing. After 30 days, the parents with similar growth conditions and 24 seedlings of each line were selected for transplantation. All rice materials were pla...

Embodiment 2

[0043] Example 2 Molecular marker-assisted selection

[0044] Molecular markers IndelWbph-1 and molecular markers IndelWbph-2 were set at the upstream and downstream of the QTL locus qWBPH-3, respectively, and primers were designed;

[0045] The primer pair for molecular marker Indel Wbph-1 is:

[0046] Upstream primer: 5'-GGCTATTATGTACACTGCAGCA-3', SEQ ID NO.1;

[0047] Downstream primer: 5'-CTGGATCGGCAGGCGTAG-3', SEQ ID NO.2;

[0048] The primer pair for molecular marker Indel Wbph-2 is:

[0049] Upstream primer: 5'-TCGAACCGCTCCATCCATTT-3', SEQ ID NO.3;

[0050] Downstream primer: 5'-TTGGCCATGGAAGGAGGG-3', SEQ ID NO.4.

[0051] Genomic DNA was extracted from the rice leaves of parents Reyan 2, Huazhan and its F1 generation and RIL population, and PCR amplification was performed on the genomic DNA using the above molecular markers;

[0052] PCR reaction system: 1 μL of upstream primer (10 μmol), 1 μL of downstream primer (10 μmol), 2 μL of DNA template (>100 ng / μL), 6 μL o...

Embodiment 3

[0057] Example 3 Application of QTLs related to rice white-backed planthopper resistance in rice breeding

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Abstract

The invention discloses a major QTL for regulating and controlling the resistance of sogatella furcifera, and belongs to the technical field of rice breeding and molecular biology, the QTL is locatedon chromosome 3 of rice, the genetic distance is 55.98-62.91 cM, and the physical distance is 13058915-14674834 bp; and the invention also discloses a molecular marker closely linked with the QTL. TheQTL and the molecular marker thereof are used for breeding rice with sogatella furcifera resistance, so that the screening efficiency can be improved.

Description

technical field [0001] The invention relates to the technical fields of rice breeding and molecular biology, and more specifically relates to a main effect QTL for regulating rice white-backed planthopper resistance, a molecular marker and its application. Background technique [0002] Rice is one of the most important food crops in the world, and a sustained and steady increase in rice production is an important guarantee for world food security. However, rice production suffers from various pests all year round, among which the white-backed planthopper (Sogatella furcifera Horváth) is one of the main pests that endanger rice production. [0003] The white-backed planthopper is a seasonal migratory pest of the Homoptera planthopper family. It damages rice by piercing and sucking the phloem juice, affecting the yield and quality of rice. In severe cases, it causes tissue necrosis, plant lodging, and death. . In addition, the white-backed planthopper is also the main carrie...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/13C12Q2600/156
Inventor 饶玉春叶涵斐王跃星芦涛戴高兴周维永杨凯如杨丽君吴思思胡祎铭
Owner ZHEJIANG NORMAL UNIVERSITY
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