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Inorganic-organic composite living cell scaffold as well as preparation method and application thereof

A living cell and organic technology, applied in the field of inorganic-organic composite bioink-based living cell scaffold and its preparation, inorganic-organic composite living cell scaffold and its preparation, can solve secondary wounds, insufficient donor sources, Immunological rejection and other issues, to achieve the effects of enhanced contact, good proliferation, and freedom of migration

Active Publication Date: 2021-03-02
中科硅肤康(济宁)医疗器械科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the best clinical treatment is autologous transplantation or allogeneic transplantation, but autologous transplantation often has the limitations of insufficient donor sources and secondary wounds; allogeneic transplantation has immune rejection

Method used

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  • Inorganic-organic composite living cell scaffold as well as preparation method and application thereof
  • Inorganic-organic composite living cell scaffold as well as preparation method and application thereof
  • Inorganic-organic composite living cell scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] SrSiO 3 Preparation of microparticles:

[0060] Weigh 2.116g Sr(NO 3 ) 2 and 2.842g Na 2 SiO 3 9H 2 O in two beakers, respectively measure 50mL deionized water into the two beakers, stir until the crystals are completely dissolved; use a dropper to dissolve the Sr(NO 3 ) 2 The solution was slowly added dropwise to Na 2 SiO 3 In the solution, stir while adding dropwise until the two are completely mixed; measure about 50mL of absolute ethanol and pour it into it, seal the mouth of the cup, and stir for more than 30min; pour the mixture into a 50mL polytetrafluoroethylene hydrothermal reaction kettle, put Oven, hydrothermal reaction at 160°C for 8 hours; after the hydrothermal treatment is over, pour off the supernatant after the solution is cooled, collect the white precipitate in the lower layer, wash it with deionized water for 3 times, ultrasonic for 10 min each time, wash with absolute ethanol for 3 times, and ultrasonically for each time 10min; put the powd...

Embodiment 2

[0063] Preparation of SS-GAM inorganic-organic composite bioink

[0064] Weigh 0.7g of gellan gum powder into a jar, add 25mL of sterilized deionized water, put it in a water bath and stir at 90°C for more than 30min until the powder is completely dissolved; turn off the heating switch and let the solution naturally Cool down; when the temperature of the solution drops below 84°C, weigh 0.4g high-viscosity sodium alginate, 0.7g methylcellulose and a certain amount (0g, 0.014g, 0.035g, 0.07g four kinds of content) SrSiO 3 Add micron particles into it, stir continuously to make it fully mixed; the temperature of the high-viscosity fluid to be mixed is lowered to room temperature, and irradiated with ultraviolet light for about 1 hour to obtain sterile GAM, 2SS-GAM, 5SS-GAM, 10SS-GAM Four kinds of gradient SrSiO 3 content of inorganic-organic composite bioinks.

[0065] figure 2 It was shown that GAM hydrogel is the product of physical combination of gellan gum, sodium algina...

Embodiment 3

[0067] Preparation of SS-GAM Inorganic-Organic Composite Scaffold for Living Cells

[0068] Step 1: Preparation of gradient SS-GAM inorganic-organic composite bioink

[0069] Weigh 0.7g of gellan gum powder into a jar, add 25mL of deionized water, put it in a water bath and stir at 90°C for more than 30min until the powder is completely dissolved; turn off the heating switch and let the solution cool down naturally; When the temperature of the solution drops below 84°C, weigh 0.4g of high-viscosity sodium alginate, 0.7g of methylcellulose and a certain amount (0g, 0.014g, 0.035g, 0.07g four gradient contents) of SrSiO 3 Add micron particles into it, stir continuously to make it fully mixed; the temperature of the high-viscosity fluid to be mixed is lowered to room temperature, and irradiated with ultraviolet light for about 1 hour to obtain sterile GAM, 2SS-GAM, 5SS-GAM, 10SS-GAM Four kinds of gradient SrSiO 3 content of inorganic-organic composite bioinks.

[0070] Step 2:...

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Abstract

The invention discloses an inorganic-organic composite living cell scaffold as well as a preparation method and application thereof. The living cell scaffold comprises a scaffold integral frame constructed by inorganic-organic composite biological ink and a plurality of cells which are distributed in a layered manner in a three-dimensional space of the scaffold integral frame; the upper half partof the living cell scaffold is in an (A-C)*y layer-by-layer alternate arrangement mode, the A is bio-ink, and the C is scaffold upper-layer cells; the lower half part is in a (A-B)*x layer-by-layer alternate arrangement mode, the A is bio-ink, and the B is scaffold lower-layer cells; and the x and the y represent the cycle periods of alternate arrangement and are both positive integers. The inorganic-organic composite living cell scaffold can simulate the physiological structure of the dermis layer of human skin containing blood vessels, promote blood vessel regeneration and reconstruction ofthe dermis structure so as to accelerate skin regeneration, and has potential application value for treatment of skin full-thickness injuries and difficult-to-heal wounds.

Description

technical field [0001] The invention relates to an inorganic-organic composite living cell scaffold and its preparation method and application, in particular to an inorganic-organic composite bioink-based living cell scaffold with multi-cell layered distribution and its preparation method and application, belonging to biological technology field. Background technique [0002] Burns, lacerations, and diabetic ulcers in daily life may cause deep and difficult-to-heal skin injuries, which are often accompanied by severe damage to skin blood vessels, making it difficult for the human body to heal itself. At present, the best clinical treatment is autologous transplantation or allogeneic transplantation, but autologous transplantation often has the limitations of insufficient donor sources and secondary wounds; allogeneic transplantation has immune rejection. Based on the above, it has become an urgent need to use artificial methods to prepare living skin tissue substitutes that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38A61L27/02A61L27/54A61L27/52
CPCA61L27/60A61L27/3804A61L27/3808A61L27/025A61L27/54A61L27/52A61L2300/102A61L2300/412
Inventor 吴成铁马景阁
Owner 中科硅肤康(济宁)医疗器械科技有限公司
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