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Mass spectrum method based on droplet extraction

A mass spectrometry analysis and extraction technology, applied in the field of cell analysis, can solve problems such as difficult to meet the needs of scientific research and application, low efficiency, large number of samples, etc., to achieve improved analysis accuracy and efficiency, high analysis efficiency, and low operating costs Effect

Active Publication Date: 2021-03-05
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the detection of trace sample mass spectrometry is manual operation, the efficiency is extremely low, and the number of samples is huge, which is difficult to meet the needs of scientific research and application. There is an urgent need for a set of methods that can release hands, improve work efficiency, and intelligent analysis.

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  • Mass spectrum method based on droplet extraction
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Embodiment 1

[0031] figure 1 The flow chart of the mass spectrometry method based on droplet extraction in the embodiment of the present invention is given, such as figure 1 Shown, described mass spectrometry analysis method based on droplet extraction comprises the following steps:

[0032] (A1) Obtain the mass spectrum signal output by the mass spectrometer as the background signal when no sample is injected;

[0033] (A2) generating a single droplet and applying it to the sample to be tested; the sample to be tested is extracted by the single droplet;

[0034] (A3) Sampling the extracted substance of the sample to be tested;

[0035] (A4) The sampled extract is ionized and sent to a mass spectrometer for analysis;

[0036] (A5) removing the background signal from the mass spectrum signal output by the mass spectrometer to obtain a difference signal;

[0037] If the intensity of the characteristic peak in the difference signal exceeds the threshold, it indicates that the mass spectro...

Embodiment 2

[0072] An application example of the mass spectrometry method based on droplet extraction in single cell analysis according to Example 1 of the present invention.

[0073] In this application example, the inner and outer walls of the capillary are treated as follows: soak the capillary in 8%~12% NaOH solution and Piranha solution for 2~10 minutes each, and then soak the capillary in 18%~22% NaOH solution. Put in the ethanol solution of chlorodimethylsilane for 1~2 hours, wash it with clean water, and then place the capillary in an oven at 100~150 degrees to bake for 30~60 minutes in order to remove the stress.

[0074] The surface treatment method of the cell carrier is as follows:

[0075] Mix the silica sol and the sulfonic acid-modified nano-silica solution in a certain ratio (preferably 1:16), coat the surface of the cell carrier, and bake in an oven at 100-150 degrees for 30-60 minutes; the cells Slide coefficient of the surface of the carrier .

[0076] Use the laser...

Embodiment 3

[0113] According to the application example of the mass spectrometry method based on droplet extraction in single cell analysis in Example 1 of the present invention, the difference from Example 2 is:

[0114] The way to generate a single droplet is:

[0115] (C1) Establish a mapping relationship between the volume of a single droplet and capillary parameters, the gas pressure pushing the liquid in the capillary, and the capillary lift velocity:

[0116] , V is the drop volume, R is the inner diameter of the capillary bottom, is the taper at the bottom end of the capillary, is the viscosity of the liquid, is the density of the liquid, is the acceleration of gravity, is the surface tension of the liquid, is the lift velocity of the capillary, is the distance from the liquid level in the capillary to the bottom end of the capillary, is the gas pressure pushing the liquid in the capillary, is the maximum value of the angle between the generatrix of the inner su...

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Abstract

The invention provides a mass spectrum analysis method based on droplet extraction. The method comprises the following steps: (1) acquiring a mass spectrum signal output by a mass spectrometer as a background signal when no sample is injected; (A2) generating a single liquid drop, and applying the single liquid drop to a to-be-detected sample, wherein the to-be-detected sample is extracted by thesingle liquid drop; (A3) sampling the extraction substance of the to-be-detected sample; (A4) ionizing the sampled extraction substance, and sending the ionized extraction substance to a mass spectrometer for analysis; (A5) removing the background signal from the mass spectrum signal output by the mass spectrometer to obtain a difference signal, if the intensity of the characteristic peak in the difference signal exceeds a threshold value, making the mass spectrometer perform sampling, and executing a step (A6), and if the intensity of the characteristic peak in the difference value signal does not exceed the threshold value, making the mass spectrometer do not carry out sampling, and executing a step (A7); (A6) analyzing the difference signal so as to identify the to-be-detected sample; and (A7) stopping ionization. The method has the advantages of high precision, high analysis efficiency and the like.

Description

technical field [0001] The present invention relates to cell analysis, in particular to a mass spectrometry method based on droplet extraction. Background technique [0002] Trace samples include but are not limited to single cells, tiny regions in tissue sections, planar sediments, etc., and their research is of great significance to analytical chemistry itself. Analytical chemistry itself shoulders the mission of helping people understand the microcosm. From organ tissue imaging to single-cell imaging and even single-molecule detection, the samples of analytical chemistry research are getting smaller and smaller, the sensitivity of analytical methods is getting higher and higher, and people's understanding of the microscopic world is getting deeper and deeper. Therefore, the realization of analysis and detection for smaller volume samples is the constant pursuit of analytical chemistry research. [0003] Mass spectrometry is a method for simultaneous analysis of multiple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/72
Inventor 闻路红甘剑勤刘云余晓梅陈安琪洪欢欢李文
Owner NINGBO UNIV
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