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Method for identifying prunella vulgaris honey and application thereof

A technology of Prunella vulgaris and honey, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., and can solve the problems of limited research on medicinal plant honey

Pending Publication Date: 2021-03-09
河南汇泉堂中药饮片科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, most of the research is on the evaluation of the chemical components and nutritional functions in the characteristic honey, and the research on the medicinal plant honey is still very limited.

Method used

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  • Method for identifying prunella vulgaris honey and application thereof
  • Method for identifying prunella vulgaris honey and application thereof
  • Method for identifying prunella vulgaris honey and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This embodiment provides a method for identifying Prunella vulgaris honey.

[0056] 1. Sample processing

[0057] (1) Dissolve Prunella vulgaris honey in water to form Prunella vulgaris honey solution.

[0058] (2) the Prunella vulgaris honey solution obtained in step (1) is passed through the activated solid-phase extraction column to absorb the active ingredients in the honey;

[0059] (3) The elution column obtained in step (2) with active ingredients adsorbed is first rinsed twice with pure water, then eluted with methanol and collected the eluent;

[0060] (4) Concentrate the eluate obtained in step (3), remove the methanol to obtain a solid, and redissolve the obtained solid in methanol to obtain an injection solution.

[0061] 2. Detection by liquid chromatography-quadrupole-time-of-flight mass spectrometry

[0062] The injection solution was detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry, and the experimental conditions were as ...

Embodiment 2

[0073] In this example, the characteristic ions 307.0824, 361.0697 and 425.1089 obtained in Example 1 are practically applied. Set up a gradient experiment group, take 0.05g, 0.25g, 0.5g, 1g and 5g of Prunella vulgaris honey and dissolve them in water to obtain experimental groups 1-5; Vitex honey, jujube flower honey and rapeseed honey were added to obtain experimental groups 6-9.

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Abstract

The invention relates to a method for identifying prunella vulgaris honey and application thereof. The method comprises the following steps of detecting to-be-detected honey by liquid chromatography-tandem mass spectrometry, and if characteristic ion peaks of m / z 307.0824, 361.0697 and 425.1089 are detected in a negative ion mode, determining that the to-be-detected honey contains prunella vulgaris honey. According to the method, the characteristic ion peaks m / z307.0824, 361.0697 and 425.1089 are screened by performing mass spectrometry on the solid-phase extract of the selfheal honey, and further experimental verification finds that only the selfheal honey can detect the characteristic peaks of the three ions, so detection sensitivity of the selfheal honey is greatly improved; therefore,the method for identifying prunella vulgaris honey, which is used for detecting m / z 307.0824, 361.0697 and 425.1089 characteristic ions through liquid chromatography-tandem mass spectrometry, is determined, and has important significance for identifying the variety of prunella vulgaris honey.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a method for identifying Prunella vulgaris honey and its application. Background technique [0002] Honey is an important bee product not only as a traditional tonic and natural sweetener, but also to stimulate wound healing, tissue regeneration, and relief from gastrointestinal disorders, gingivitis, and other ailments. The composition, taste, and color of different varieties of honey depend on the type of nectar source, geographical location, climate, and bee species that collect honey, and are also affected by processing technology and storage conditions. In the prior art, the chemical components and nutritional functions in characteristic honey are mostly evaluated and studied, and the research on medicinal plant honey is still very limited. [0003] Prunella vulgaris is a plant of the Labiatae family and is a widely planted Chinese herbal medicine. It has the effects of clearing...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 刘鹏
Owner 河南汇泉堂中药饮片科技有限公司
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