Method for identifying prunella vulgaris honey and application thereof
A technology of Prunella vulgaris and honey, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., and can solve the problems of limited research on medicinal plant honey
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Embodiment 1
[0055] This embodiment provides a method for identifying Prunella vulgaris honey.
[0056] 1. Sample processing
[0057] (1) Dissolve Prunella vulgaris honey in water to form Prunella vulgaris honey solution.
[0058] (2) the Prunella vulgaris honey solution obtained in step (1) is passed through the activated solid-phase extraction column to absorb the active ingredients in the honey;
[0059] (3) The elution column obtained in step (2) with active ingredients adsorbed is first rinsed twice with pure water, then eluted with methanol and collected the eluent;
[0060] (4) Concentrate the eluate obtained in step (3), remove the methanol to obtain a solid, and redissolve the obtained solid in methanol to obtain an injection solution.
[0061] 2. Detection by liquid chromatography-quadrupole-time-of-flight mass spectrometry
[0062] The injection solution was detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry, and the experimental conditions were as ...
Embodiment 2
[0073] In this example, the characteristic ions 307.0824, 361.0697 and 425.1089 obtained in Example 1 are practically applied. Set up a gradient experiment group, take 0.05g, 0.25g, 0.5g, 1g and 5g of Prunella vulgaris honey and dissolve them in water to obtain experimental groups 1-5; Vitex honey, jujube flower honey and rapeseed honey were added to obtain experimental groups 6-9.
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