Method for identifying prunella vulgaris honey and application thereof

A technology of Prunella vulgaris and honey, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., and can solve the problems of limited research on medicinal plant honey

Pending Publication Date: 2021-03-09

河南汇泉堂中药饮片科技有限公司

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AI-Extracted Technical Summary

Problems solved by technology

In the prior art, most of the research is on the evaluation of the chemical components and nutritional fu...

Abstract

The invention relates to a method for identifying prunella vulgaris honey and application thereof. The method comprises the following steps of detecting to-be-detected honey by liquid chromatography-tandem mass spectrometry, and if characteristic ion peaks of m/z 307.0824, 361.0697 and 425.1089 are detected in a negative ion mode, determining that the to-be-detected honey contains prunella vulgaris honey. According to the method, the characteristic ion peaks m/z307.0824, 361.0697 and 425.1089 are screened by performing mass spectrometry on the solid-phase extract of the selfheal honey, and further experimental verification finds that only the selfheal honey can detect the characteristic peaks of the three ions, so detection sensitivity of the selfheal honey is greatly improved; therefore,the method for identifying prunella vulgaris honey, which is used for detecting m/z 307.0824, 361.0697 and 425.1089 characteristic ions through liquid chromatography-tandem mass spectrometry, is determined, and has important significance for identifying the variety of prunella vulgaris honey.

Application Domain

Component separation

Technology Topic

Tandem mass spectrometrySolid phase extraction +5

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Examples

Experimental program(2)
Effect test(1)

Example Embodiment

[0054] Example 1

[0055] This embodiment provides a method of identifying a summer obscener honey.

[0056] 1, the treatment of the sample

[0057] (1) Take the summer Blus honey in water to form a micro-grass honey solution.

[0058] (2) Pharained step (1) The subunited honey solution obtained by activating the column, adsorb the effective ingredients in honey;

[0059] (3) The adsorption of the adsorption of step (2) has an effective component, first eluting with pure water, then eluting with methanol and collecting eluent;

[0060] (4) Concentrate the eluent obtained by step (3), and the solid obtained is removed after removal of methanol, and the obtained solid matter is binding to methanol, i.e., the solution is obtained.

[0061] 2, liquid chromatography-quadrupole-flight time mass spectrometry

[0062] Testing the injection solution with liquid chromatography - quadrupole - flight time mass spectrometry, the experimental conditions are as follows:

[0063] Liquid Chromatography:

[0064] The chromatographic column is a C18 column; the flow phase A is 0.1% formic acid aqueous solution, the flow phase B is methanol; the gradient elution procedure is: 0-1 minutes of the volume fraction of 0-1 minutes increase to 5%, 1-6 min mobile phase B The volume fraction increased from 5% to 55%, and the volume fraction of 6-20 min mobile phase B was increased from 55% to 95%, and the volume fraction of 20-26 min mobile phase B was maintained at 95%, 26-27 min mobile phase B volume fraction. Falling to 5%.

[0065] Quadrupole - flight time mass spectrometry:

[0066] ESI, negative ion mode; dry gas temperature: 310 ~ 330 ° C; dry air flow rate: 7 ~ 9L / min; sphing gas temperature: 340 ~ 360 ° C; sphing gas flow rate: 10 ~ 12L / min; spray gas pressure: 38 ~ 42 psi Capillary voltage: 3400 ~ 3600V; nozzle voltage: 800 ~ 1100V.

[0067] Get it after test figure 1 The desired ion chart, the present embodiment is further compared with the composition of the summer crane flowers, and 3 feature peaks are obtained from 307.0824, 361.0697, 425.1089 (allowed deviation ± 5 ppm), respectively.

[0068] At the same time, the present embodiment uses the same detection method of the eucalyptus honey, the thoroughness honey, jujube honey and rapeseed, and the above-mentioned test method - quadrupole - flight time mass spectrometry, Figure 2- Figure 5 Test results

[0069] 3, characteristic ion analysis

[0070] This embodiment further uses the above to liquid chromatography-quadrupole rod - flight time mass spectrometry for the summer blurate honey, eucalyptus honey, thorns honey, jujube honey and rape honey, respectively, in terms of feature ions 307.0824, 361.0697, 425.1089, respectively, Obtain Figure 6 - Figure 20 The result shown.

[0071] These chromatograms are analyzed, and only the chromatogram of the bruised honey is shown in Characterization ions 307.0824, 361.0697, 425.1089, which are not extracted in the chromatogram of other four honey. This shows the above detection of the eucalyptus honey, the jujube honey, jujube honey and the rapeseed honey, and cannot be detected by ion 307.0824, 361.0697 and 425.1089.

Example Embodiment

[0072] Example 2

[0073] The present embodiment performs practical application of the feature ions 307.0824, 361.0697, and 425.1089 obtained in Example 1. To set the gradient experiment group, take 0.05g, 0.25g, 0.5g, 1g and 5g of Xia Blood honey in water to be dissolved in the experimental group 1-5; 5g of Xia Blood honey is dissolved in water, then take 1g of summer tanket honey, Those honey, jujube honey and rapeseed honey were added to the experimental group 6-9.

PUM

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