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Picea koraiensis tissue culture medium and culture method

A technology of tissue culture medium and tissue culture, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low yield and low reproduction rate, and achieve the effects of promoting absorption, promoting high-efficiency, and promoting efficiency

Pending Publication Date: 2021-03-12
苏州梵时轮园艺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of the prior art, to provide a red spruce tissue culture medium and its cultivation method, which solves the problems of low reproduction rate and low yield in traditional seed propagation, cutting propagation and other modes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1. Select dormant buds, needles, tender stems and other parts of red bark spruce as explants, and disinfect them:

[0030] Take dormant buds as explants: take dormant buds as explants in the winter of December.

[0031] Disinfection treatment: After the dormant buds are rinsed with clean water, they are sent to a sterile ultra-clean workbench, soaked in 75% alcohol for 30 seconds, quickly immersed in sterile water, and then rinsed with flowing sterile water for 5 times for pretreatment and disinfection. Then use 10% NaClO solution (also containing 1% Tween-20, 2% 84 disinfectant) to soak for 3 minutes for in-depth disinfection. Then soak in 5% chlorothalonil for 30 minutes, and then soak in 1% potassium permanganate solution for 3 minutes. Potassium permanganate not only has a bactericidal function, but studies have found that this step helps to improve the efficiency of subsequent callus formation. Rinse with sterile water 5 times for subsequent operations.

[...

Embodiment 2

[0046] Step 1. Select dormant buds, needles, tender stems and other parts of spruce spruce as explants, and disinfect them: the steps are the same as in Example 1.

[0047] Step 2, pretreatment of explants before induction: the steps are the same as in Example 1.

[0048] Step 3, preparation of callus induction culture:

[0049] The specific formula of callus induction medium is as follows: 1 / 2 LM medium + 2,4-D (0.4mg / L) + 6-BA (1.0 mg / L) + NAA (0.3mg / L) + yellow-based salicylus acid (0.1 μg / L) + sodium pyruvate (0.7 μg / L) + β-mercaptoethanol (final concentration 1:2000) + Vorinostat (SAHA, a histone deacetylase inhibitor, final concentration 0.26 μmol / L)+Trichostatin A (TSA, a histone deacetylase inhibitor, final concentration 20 nmol / L)+ 5-azacytidine (5-AZA, a DNA methylase inhibitor 2μmol / L) + Triton-100 (0.01%).

[0050] Inoculate the explants treated in steps 1 and 2 into the culture medium. One week after the dormant buds were inoculated, green granular protrusion...

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Abstract

The invention discloses a picea koraiensis tissue culture medium and a culture method. The method comprises the following steps: selecting picea koraiensis dormant buds, needles or tender stems as explants, and performing disinfection treatment; pretreating the explants before induction, and soaking the explants in an 8g / L NaCl solution for 3-5 minutes; performing callus tissue induction; performing proliferation of callus tissue; and inducing differentiation of buds. Based on the superiority of tissue culture, the method provided by the invention can solve the problems of low reproductive rate, low yield and the like of traditional manners of seed propagation, cutting propagation and the like to the greatest extent, and realizes the tissue culture of picea koraiensis; in addition, the success rate of taking dormant buds as the explants is the highest; and besides, the possibility of taking needles, tender stems and the like as the explants is also provided, so that the tissue cultureof the picea koraiensis is not only limited to picking the dormant buds in winter, but induction can still be performed by picking the needles and the tender stems in spring.

Description

technical field [0001] The invention relates to a red bark spruce tissue culture medium and a culture method, belonging to the technical field of plant cultivation. Background technique [0002] The spruce plant is tall and straight, with soft wood and few knots, uniform and beautiful texture, neat structure and easy processing, and good resonance ability. It also plays an important role in papermaking materials and afforestation. However, the propagation efficiency of adult Picea plants by cuttings or seeds is very low, and tissue culture is difficult and induction efficiency is low. The difficulty of breeding caused by the above reasons has greatly restricted the large-scale application of spruce as an excellent coniferous tree. Contents of the invention [0003] The purpose of the present invention is to overcome the defects of the prior art, provide a red bark spruce tissue culture medium and a culture method, and solve the problems of low reproduction rate and low yi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 卢鹏
Owner 苏州梵时轮园艺科技有限公司
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