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Application of inhibitor of parvalbumin in preparation of product for preventing and treating obesity and diseases caused by obesity

A parvalbumin and inhibitor technology, applied in the field of medicine, can solve the problems of large molecular weight, difficult synthesis, poor stability, etc., and achieve the effect of improving insulin resistance

Active Publication Date: 2021-03-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these secreted proteins have the problems of large molecular weight, difficult synthesis and poor stability, thus limiting their clinical application.

Method used

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  • Application of inhibitor of parvalbumin in preparation of product for preventing and treating obesity and diseases caused by obesity
  • Application of inhibitor of parvalbumin in preparation of product for preventing and treating obesity and diseases caused by obesity
  • Application of inhibitor of parvalbumin in preparation of product for preventing and treating obesity and diseases caused by obesity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Establishment of mouse obesity model and body weight change after polypeptide injection

[0057] Establishment of the mouse obesity model: the obesity model was established by feeding the mice a diet containing 60% high-fat for 12 weeks. After the model is established successfully, follow-up body weight detection and examples are carried out.

[0058] The mice were injected once a day with 5 mg / kg of polypeptide or normal saline as a control, and the body weight of the mice was weighed. The result is as figure 1 As shown, the body weight of mice decreased significantly.

Embodiment 2

[0059] Embodiment 2 detects the situation of mouse fatty liver

[0060] Seven days after the injection of the polypeptide, the mouse liver was taken for paraffin section, and the condition of fatty liver was observed by oil red staining.

[0061] Materials: Mice were killed by cervical dislocation, the abdominal cavity was opened, and liver tissue (or adipose tissue) was cut. The cut tissue block should not be too large to allow the fixative to penetrate, usually 5mm×5mm×2mm or 10mm×10mm×2mm is appropriate. Remove the required liver tissue and cut it into a small piece with a thickness of 2-3 mm.

[0062] Fixation: Wash the cut tissue with normal saline, put it into Carnot's fixative immediately and fix it for 30-50 minutes.

[0063] Washing: After the material is fixed, wash it with 50%-70% ethanol.

[0064] Dehydration: 30%, 50%, 70%, 80%, and 90% ethanol solutions at different levels were dehydrated for 40 minutes each, and put in 95%, 100% ethanol solutions on both side...

Embodiment 3

[0097] Example 3 Detection of Insulin and Triglyceride Levels

[0098] Seven days after the injection of the polypeptide, the mice were killed and the serum of the mice was taken to detect the levels of insulin and triglycerides by enzyme-linked immunosorbent assay (ELISA for short). The ELISA experimental steps are as follows:

[0099] 1. Coating process:

[0100] Dilute the antigen used with coating diluent to an appropriate concentration (generally, the amount of antigen coating required is 20-200 μg per well), add 100 μl of antigen to each well, place at 37°C for 4h, or 4°C for 24h; discard the well Liquid. (To avoid evaporation, plates should be covered or placed flat in a metal wet box with wet gauze on the bottom)

[0101] 2 Seal the enzyme-labeled reaction well:

[0102] Place 5% calf serum at 37°C for 40 min. When blocking, fill the reaction wells with blocking solution and remove the air bubbles in each well. After sealing, wash the wells with washing solution for ...

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Abstract

The invention relates to the field of medicine, in particular to application of parvalbumin in preparation of product for preventing and treating obesity and diseases caused by obesity. The parvalbumin inhibitor is selected from a substance capable of being specifically combined with small albumin or a substance capable of being specifically combined with a small albumin target. The parvalbumin inhibitor is selected from polypeptide, and the amino acid sequence of the polypeptide is shown as SEQ ID NO. 1. The inventor of the invention finds the function and action mechanism of the parvalbumininhibitor in obesity, inhibits the combination of the parvalbumin inhibitor and the colony stimulating factor 1 receptor by designing the synthetic polypeptide, and plays a role in reducing weight andimproving insulin tolerance, fatty liver and other symptoms caused by obesity in high-fat induced obesity mice. A new theoretical basis is laid for weight reduction and obesity-related disease treatment, and a new drug action target is provided.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of an inhibitor of parvalbumin in the preparation of products for the prevention and treatment of obesity and diseases caused by obesity. Background technique [0002] Obesity not only affects people's physical beauty, but is also closely related to a series of major diseases, such as type 2 diabetes, cardiovascular disease, fatty liver, cancer, etc. It has been listed by the World Health Organization as one of the top ten diseases that threaten human health. [0003] Adaptive thermogenesis has become one of the current research hotspots because of its important role in the fight against obesity and type 2 diabetes. Brown adipose tissue (Brown adipose tissue), which is widespread in mammals, is an important tissue for adaptive thermogenesis. Brown adipose tissue expresses uncoupling protein-1 (UCP1), a thermogenic protein located on the inner mitochondrial membrane. UCP1 causes ...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61P3/04A23L33/18
CPCA61K38/10A61P3/04A23L33/18A23V2002/00A23V2200/332A23V2250/55
Inventor 栾冰林绍坚张安可袁玲
Owner TONGJI UNIV
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