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circRNA detection kit for auxiliary diagnosis of autism

A technology for auxiliary diagnosis and autism, applied in the field of RNA detection, can solve the problem of autism, and achieve the effect of great clinical value and socio-economic significance

Active Publication Date: 2021-03-12
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no early and objective method for the diagnosis of autism

Method used

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  • circRNA detection kit for auxiliary diagnosis of autism
  • circRNA detection kit for auxiliary diagnosis of autism
  • circRNA detection kit for auxiliary diagnosis of autism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Extraction of total RNA in plasma or serum of autistic patients by Trizol method

[0032] 1) Pipette 300 μL plasma or serum into a new EP tube, add 900 μL Trizol, and let stand at room temperature for 5 minutes;

[0033] 2) Add 300 μL chloroform, shake vigorously for 15 seconds, and let stand at room temperature for 5 minutes;

[0034] 3) High-speed centrifugation at 12,000 rpm for 15 minutes at 4°C;

[0035] 4) Transfer the upper layer of plasma or serum to another clean EP tube (the oil below the stratification cannot be absorbed), add 500 μl of isopropanol, and place it in a -20°C refrigerator for 10 minutes;

[0036] 5) High-speed centrifugation at 12,000pm for 10min at 4°C;

[0037] 6) Discard the supernatant, add 1ml pre-cooled 75% CH 3 CH 2 OH;

[0038] 7) Centrifuge at 7,500mpm for 5min at 4°C; discard the supernatant, and dry at room temperature for 10-30min (do not completely air-dry or incompletely air-dry);

[0039] 8) Add 20 μL of DEPC-treated wat...

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Abstract

The invention discloses a circRNA detection kit for auxiliary diagnosis of autism, and belongs to the technical field of RNA detection. According to the kit for auxiliary diagnosis of autism providedby the invention, total RNA of a to-be-detected sample is extracted, circRNA reverse transcription is carried out, qPCR is carried out on an obtained reverse transcription product, so that relative expression of target circRNA is detected, and rapid, sensitive and specific detection of an autism marker hsa_circ_0004566 is successfully achieved. The kit for auxiliary diagnosis of autism provided bythe invention can detect autism markers objectively in a non-invasive (brain tissue) and biological manner, can play a role in early identification of autism children patients and auxiliary diagnosisof autism patients and in-time intervention and treatment, and has important clinical value and social and economic significance.

Description

technical field [0001] The invention relates to the technical field of RNA detection, in particular to a circRNA detection kit for auxiliary diagnosis of autism. Background technique [0002] Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders that seriously affect children's physical and mental health. The children are mainly manifested in different degrees of language barriers, social barriers, narrow interests, and stereotyped behaviors. More onset in infancy. At present, autism has become the main cause of disability for children under the age of 6 in my country, seriously endangering children's physical and mental health and bringing heavy economic and spiritual burdens to society and families. [0003] At present, the methods of clinical diagnosis of ASD in the world are mainly based on subjective observation and evaluation of children's speech and behavior. Autism Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Autism Diagnostic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/178C12Q2531/113C12Q2545/101C12Q2561/113C12Q2563/107
Inventor 崔立华杜文冉冯福民
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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