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A method for doubling cucumber haploids

A haploid, cucumber technology, applied in the field of plant tissue culture, can solve the problems of long doubling process, dead seedlings, damage to regenerated plants, etc., and achieves the effect of improving the doubling rate of chromosomes and saving labor.

Active Publication Date: 2022-02-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the colchicine-induced doubling method has a complicated operation process, takes a long time for the doubling process, and causes damage to the regenerated plants, resulting in dead seedlings.
There are related reports about the natural doubling of cucumber haploid culture regenerated plants, but only for a specific genotype of cucumber material (such as Chinese thorny cucumber), and the specific method is unknown

Method used

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  • A method for doubling cucumber haploids
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Embodiment 1

[0038] A method for doubling cucumber haploids:

[0039] 1) Embedding the radicle of the cucumber embryoid body in the regeneration medium and carrying out the first cultivation to obtain the cucumber seedling with the first complete true leaf; the condition of the first cultivation: the light intensity during the day is 4000LUX, and the temperature during the day is 26°C; culture in dark at night, the temperature at night is 22°C;

[0040] The regeneration medium is based on MS medium, which is 3% sucrose, 0.8% agar, 0.2mg / L of 6-benzylaminopurine and 0.05mg / L of naphthaleneacetic acid;

[0041] 2) The cucumber seedlings with the first complete true leaf obtained in step 1) are subjected to a second culture to realize doubling of the cucumber haploid; the conditions for the second culture: the light intensity during the day is 7000LUX, and the temperature during the day is 26°C; Cultivate in the dark at night, and the temperature at night is 22°C.

[0042] Cucumbers are den...

Embodiment 2

[0044] A method for doubling cucumber haploids:

[0045] 1) Embedding the radicle of the cucumber embryoid body in the regeneration medium and carrying out the first cultivation to obtain the cucumber seedling with the first complete true leaf; the condition of the first cultivation: the light intensity during the day is 4000LUX, and the temperature during the day is 26°C; culture in dark at night, the temperature at night is 22°C;

[0046] The regeneration medium is based on MS medium, which is 3% sucrose, 0.8% agar, 0.2mg / L of 6-benzylaminopurine and 0.05mg / L of naphthaleneacetic acid;

[0047] 3) The cucumber seedlings with the first complete true leaf obtained in step 1) are subjected to a second culture to realize doubling of the cucumber haploid; conditions for the second culture: the daytime light intensity is 7000LUX, and the daytime temperature is 26°C; Cultivate in the dark at night, and the temperature at night is 22°C.

[0048] Cucumbers are European cucumbers. 5...

Embodiment 3

[0050] A method for doubling cucumber haploids:

[0051] 1) Embedding the radicle of the cucumber embryoid body in the regeneration medium and carrying out the first cultivation to obtain the cucumber seedling with the first complete true leaf; the condition of the first cultivation: the light intensity during the day is 4000LUX, and the temperature during the day is 26°C; culture in dark at night, the temperature at night is 22°C;

[0052] The regeneration medium is based on MS medium, including 3% sucrose, 0.8% agar, 0.2mg / L of 6-benzylaminopurine and 0.05mg / L of naphthaleneacetic acid;

[0053] 4) The cucumber seedlings with the first complete true leaf obtained in step 1) are subjected to a second culture to realize doubling of the cucumber haploid; conditions for the second culture: the daytime light intensity is 7000LUX, and the daytime temperature is 26°C; Cultivate in the dark at night, and the temperature at night is 22°C.

[0054] Cucumbers are South China type cucum...

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Abstract

The invention provides a method for doubling cucumber haploids, belonging to the technical field of plant tissue culture, comprising: embedding the radicles of cucumber haploid embryoids in a regeneration medium for the first culture to obtain the first complete Cucumber seedlings with true leaves; the regeneration medium includes sucrose, agar, 6-benzylaminopurine and naphthaleneacetic acid; the conditions for the first culture: the light intensity is 3000-5000LUX during the day, and the temperature is 24-28°C; the dark culture at night, the temperature is 20 ~25°C; carry out the second culture to double the cucumber haploid; the conditions for the second culture: daytime light intensity is 5000-9000LUX, temperature 24-28°C; night dark culture, temperature 20-25°C. According to the method provided by the invention, the natural doubling rate of chromosomes of the dense thorn type cucumber material can reach 84.7%, the natural chromosome doubling rate of the European type material reaches 72.7%, and the chromosome doubling rate of the South China type material reaches 71.3%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for doubling cucumber haploids. Background technique [0002] Through the cultivation of unpollinated ovary of cucumber, the haploid cells in the embryo sac can be cultured into haploid embryoid bodies, which can then be cultivated into regenerated plants, usually the chromosomal ploidy of these regenerated plants is still haploid , the regenerated haploid plant needs to undergo chromosome doubling, and the doubled haploid plant can be applied to the breeding of new cucumber varieties, and is an important breeding material in cucumber genetics and breeding. [0003] Usually the chromosome doubling of haploid plants is the most widely used chemical mutagenesis method, and the commonly used mutagen in cucumber haploid culture is colchicine. However, the colchicine-induced doubling method has a complicated operation process, takes a long time for the doubling ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 刘立功白惠平张峰于拴仓王晶赵泓李军刘士华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES