Targeted antibacterial peptide for salmonella and preparation method of targeted antibacterial peptide

Abstract

The invention discloses targeted antibacterial peptide for salmonella and a preparation method of the targeted antibacterial peptide. The targeted antibacterial peptide for the salmonella is preparedby the following steps of selecting a short peptide sequence without positive charge from a plurality of peptide fragments with salmonella affinity, which are obtained by screening a bacteriophage peptide library, wherein the sequence only has a salmonella binding function and does not have bactericidal activity; and on the basis of the affinity sequence, adding positive charge and hydrophobic amino acid on the basis of the sequence in combination with structural functional relationship research. The targeted antibacterial peptide for the salmonella can specifically kill the salmonella, guarantees that other bacteria are not affected while the salmonella is killed, enables beneficial bacteria to survive, and maintains balance of microbial structures.

Description

technical field [0001] The invention relates to the technical field of antimicrobial peptides, in particular to a targeted antimicrobial peptide against Salmonella and a preparation method thereof. Background technique [0002] Salmonella is a pathogenic bacterium that can cause salmonellosis in humans and animals. The disease is mainly manifested by enteritis and sepsis, and can be fatal in severe cases. Salmonella is mostly chronic or recessive infection in adult livestock and poultry, making the infected livestock and poultry a mobile source of infection, increasing the risk of Salmonella outbreaks in young livestock, chicks, and humans, not only causing serious economic losses to the breeding industry, It will seriously threaten human health. In developed countries, Salmonella is the number one cause of human diarrhea. With the development of my country's economy, the ranking of Salmonella has been rising among the causes of human diarrhea, ranking among the top three i...

AI Technical Summary

Problems solved by technology

However, the extensive use of broad-spectrum antibiotics has the following three disadvantages: 1. Inducing the production of drug-resistant bacteria: bacteria often become "drug-resistant" after acquiring multi-drug resistance, which seriously threatens the safety of human life; Spectrum antibiotics can also kill beneficial bacteria while killing Salmonella, and the absence of beneficial bacteria is not conducive to the health and resistance of livestock and poultry; 3. Food safety issues: antibiotics left in livestock and poultry products enter the human body and continue to grow in the human body. Induces drug-resistant bacteria and kills beneficial bacteria in the human gut

4. Ecological environmental pollution: Antibiotics entering the environment will destroy the bacterial composition in the environment and cause ecological imbalance

[0005] However, most of the existing antimicrobial peptides are still broad-spectrum antibacterial, killing other beneficial bacteria indiscriminately while killing pathogenic bacteria

The absence of beneficial bacteria is not conducive to the health and resistance of livestock and poultry

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