Method for observing epidermal hair of plant leaves by using fluorescence microscope

Abstract

The invention relates to a method for observing epidermal hair of a plant leaf by using a fluorescence microscope. The method can effectively solve the problems of clearly observing the type and distribution of the epidermal hair of the plant and ensuring the quality of medicinal materials by using the fluorescence microscope, and comprises the following steps of: cutting off mature leaves from aplant, cutting off 5-10 mm<2> leaves without thick veins on two sides of the middle section of the middle vein of each leaf, putting the cut leaves into methanol, removing chlorophyll by using ethanol, soaking in berberine, treating by using vanillin-concentrated hydrochloric acid, rinsing, sealing, observing the types and distribution of glandular hairs and non-glandular hairs by using an epifluorescence microscope, and shooting. The method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, can completely solve the problem of observation of various plants with leaf epidermis densely covered by non-glandular hairs and glandular hairs, can achieve an ideal effect by not having good requirements on an operation technology, isan innovation in plant structure observation, and has very strong practicability and remarkable economic and social benefits.

Description

technical field [0001] The invention relates to the field of microscopic observation of plants, in particular to a method for observing plant leaf epidermis with a fluorescence microscope, which uses a freehand sectioning technique to slice and dye plants. Background technique [0002] In the study of plant taxonomy, the characteristics of indumentum are a very important aspect in the study of plant leaf epidermis. The indumentum type is divided into glandular hairs and non-glandular hairs. It is generally believed that glandular hairs have a secretory effect, while non-glandular hairs only have a protective effect. For many medicinal plants, the growth and development status of leaf epidermis can directly affect the quality of medicinal materials. Therefore, in botanical research, it is often necessary to observe the type and distribution of indumentum. Yet for the multiple plants of Compositae such as mugwort, wild chrysanthemum, on the epidermis under the leaf, be dens...

AI Technical Summary

Problems solved by technology

Yet for the multiple plants of Compositae such as mugwort, wild chrysanthemum, on the epidermis under the leaf, be densely covered with a large amount of non-glandular hairs called "cotton hairs", on the one hand because of the quantity and density of "cotton hairs" it is difficult to Observation, on the other hand, due to the coverage of thick "cotton hairs", the type and distribution characteristics of the glandular hairs distributed under the "cotton hairs" cannot be seen, and it is difficult to resolve them with ordinary bright-field optical microscopes or scanning electron microscopes.