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Method for determining concentrations of dopamine and uric acid in presence of ascorbic acid

A technology for determination of ascorbic acid and concentration, applied in measuring devices, instruments, material analysis by electromagnetic means, etc., can solve problems such as affecting cell function and abnormal purine metabolism, and achieve the effect of sensitive determination

Active Publication Date: 2021-03-19
SUZHOU CHIEN SHIUNG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive uric acid content often means abnormal purine metabolism, and excessive uric acid will lead to an acidic internal environment, thereby affecting the function of cells

Method used

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  • Method for determining concentrations of dopamine and uric acid in presence of ascorbic acid
  • Method for determining concentrations of dopamine and uric acid in presence of ascorbic acid
  • Method for determining concentrations of dopamine and uric acid in presence of ascorbic acid

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Embodiment 1

[0032] A method for measuring the concentration of dopamine and uric acid in the presence of ascorbic acid, comprising the following steps:

[0033] Step 1: prepare single-layer molybdenum disulfide by known method, press single-layer molybdenum disulfide and polydiallyl dimethyl ammonium chloride mass ratio 1:20.8, the mass fraction of 200mg single-layer molybdenum disulfide and 20ml is After the 20% polydiallyldimethylammonium chloride aqueous solution is mixed evenly, press monolayer molybdenum disulfide and H 2 PtCl 6 The mass ratio is 8.9:1, add 0.55ml of 100mM H 2 PtCl 6 Solution, microwave reaction for 30 minutes, after the reaction, the product was cooled at room temperature and washed by centrifugation, stored at 4°C for later use, and functionalized molybdenum disulfide was obtained;

[0034] Step 2: Weigh 5 mg of the functionalized molybdenum disulfide from step 1 and add 10 ml of ultrapure water to dissolve it, and dissolve it by ultrasonication for 15 minutes t...

Embodiment 2

[0039] A method for measuring the concentration of dopamine and uric acid in the presence of ascorbic acid, comprising the following steps:

[0040] Step 1: prepare single-layer molybdenum disulfide by known method, press single-layer molybdenum disulfide and polydiallyl dimethyl ammonium chloride mass ratio 1: 62.4, the mass fraction of 200mg single-layer molybdenum disulfide and 60ml is After the 20% polydiallyldimethylammonium chloride aqueous solution is mixed evenly, press monolayer molybdenum disulfide and H 2 PtCl 6 The mass ratio is 8.9:1, add 0.55ml of 100mM H 2 PtCl 6 Solution, microwave reaction for 30 minutes, after the reaction, the product was cooled at room temperature and washed by centrifugation, stored at 4°C for later use, and functionalized molybdenum disulfide was obtained;

[0041]Step 2: Weigh 5 mg of the functionalized molybdenum disulfide from step 1 and add 10 ml of ultrapure water to dissolve it, and dissolve it by ultrasonication for 15 minutes t...

Embodiment 3

[0046] A method for measuring the concentration of dopamine and uric acid in the presence of ascorbic acid, comprising the following steps:

[0047] Step 1: prepare single-layer molybdenum disulfide by known method, press single-layer molybdenum disulfide and polydiallyl dimethyl ammonium chloride mass ratio 1: 41.6, 200mg single-layer molybdenum disulfide and 40ml mass fraction are After the 20% polydiallyldimethylammonium chloride aqueous solution is mixed evenly, press monolayer molybdenum disulfide and H 2 PtCl 6 The mass ratio is 8.9:1, add 0.55ml of 100mM H 2 PtCl 6 Solution, microwave reaction for 30 minutes, after the reaction, the product was cooled at room temperature and washed by centrifugation, stored at 4°C for later use, and functionalized molybdenum disulfide was obtained;

[0048] Step 2: Weigh 5 mg of the functionalized molybdenum disulfide from step 1 and add 10 ml of ultrapure water to dissolve it, and dissolve it by ultrasonication for 15 minutes to obt...

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Abstract

The invention provides a method for determining concentrations of dopamine and uric acid in the presence of ascorbic acid. The method comprises the following steps of: uniformly mixing single-layer molybdenum disulfide with a polydiallyldimethylammonium chloride water solution, adding an H2PtCl6 solution, and carrying out microwave reaction to obtain functionalized molybdenum disulfide; weighing the functionalized molybdenum disulfide, adding ultrapure water to dissolve the functionalized molybdenum disulfide; dropwise adding a functionalized molybdenum disulfide solution to the surface of a treated electrode; and airing the electrode to obtain a functionalized molybdenum disulfide modified electrode; and immersing the functionalized molybdenum disulfide modified electrode into a phosphatesolution containing dopamine, uric acid and ascorbic acid at the same time, adopting a three-electrode system, and selecting differential pulse voltammetry to realize measurement. The method can simply, conveniently and sensitively determine the concentrations of the dopamine and uric acid in the presence of the ascorbic acid.

Description

technical field [0001] The invention relates to a method for measuring concentration, in particular to a method for measuring the concentration of dopamine and uric acid in the presence of ascorbic acid. Background technique [0002] Ascorbic acid, dopamine, and uric acid exist in the extracellular fluid of the human central nervous system at the same time, and are important small molecules in the human body. Among them, dopamine and uric acid play an important role in the kidney function, cardiovascular system and central nervous system of the human body. The abnormal content of dopamine in the body will directly affect the mental activity of the human body. Excessive uric acid content often means abnormal purine metabolism, and excessive uric acid will lead to an acidic internal environment, thereby affecting the function of cells. Since dopamine, uric acid and ascorbic acid coexist in the body fluids of the organism, they tend to interfere with each other. Because its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/327Y02A50/30
Inventor 朱志强郁惠珍苗向阳
Owner SUZHOU CHIEN SHIUNG INST OF TECH
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