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Method for detecting pentacyclic triterpenoids using lc-ms APCI source

A technology for pentacyclic triterpenes and pentacyclic triterpenes is applied in the field of determination of pentacyclic triterpenes, which can solve problems such as many fragments, and achieve the effects of high sensitivity, simple pretreatment, and elimination of false positive interference.

Active Publication Date: 2021-09-17
嘉兴迈维代谢生物科技有限公司
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Problems solved by technology

The ion sources of mass spectrometry are also divided into electron bombardment ionization (EI), atmospheric pressure chemical ionization (APCI), electrospray ionization (ESI) and atmospheric pressure optical ionization (APPI); and different ion sources have different advantages and disadvantages. For example, EI may cause more fragments after fragmentation of PT, while APCI and APPI are only suitable for ionization of non-polar small molecules

Method used

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  • Method for detecting pentacyclic triterpenoids using lc-ms APCI source
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  • Method for detecting pentacyclic triterpenoids using lc-ms APCI source

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Embodiment Construction

[0035] 1. Sample pretreatment:

[0036] 1) Weigh 50±2.5mg of fresh sample, add 200μL extractant (methanol:MTBE=1:3), vortex for 5min, centrifuge at 12000r / min for 10min, after centrifugation, draw 100uL of the supernatant to a numbered glass liner Store in a sample vial at -20°C until LC-MS / MS analysis.

[0037] 2) Instruments and equipment

[0038]

[0039]

[0040] 2. Standard product processing

[0041] Dilute the purchased 3 kinds of pentacyclic triterpene standard products with MTBE / methanol (3:1) mixed solution for on-board detection to determine the ion pair information: prepare a 1ug / ml pentacyclic triterpene single standard for needle pump detection, and determine the adjustment parameters Precursor ions, product ions and optimized DP and CE, the specific information is as follows:

[0042]

[0043] 3. Optimize the chromatographic method and determine the retention time

[0044] By changing the gradient of the mobile phase and the temperature of the colum...

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Abstract

The invention provides a method for detecting pentacyclic triterpenoids by using APCI as an ion source and using LC / MS. The method does not need to be derivatized and can detect three isomers within 6 minutes ,high sensitivity.

Description

technical field [0001] The invention uses LC-MS APCI source to measure pentacyclic triterpenes, and the invention relates to the application of LC-MS, optimization of related mass spectrometry conditions and optimization of liquid chromatography conditions. Background technique [0002] Pentacyclic triterpenoids (Pentacyclic Triterpenoids, PT) compounds are an important class of natural compounds, composed of six isoprene units connected by five closed rings as the parent, mostly in free form or combined with sugar groups Glycosides are widely found in nature, especially in some traditional Chinese natural medicines. The core structure of PT is divided into five types: pneumane type, arbutane type, lupine type, hopane type and suberane type. [0003] A large number of studies have shown that pentacyclic triterpenoids have various biological activities such as anti-virus and anti-tumor and a wide range of pharmacological effects. In recent years, they have also been found to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675
Inventor 陈欣唐堂张永明郑彬
Owner 嘉兴迈维代谢生物科技有限公司
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