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Bacillus licheniformis and application thereof

A technology of Bacillus licheniformis and bacterial agent, applied to Bacillus licheniformis XCY26 and its application field, can solve the problems of long-term addition and use of feed, high price of astaxanthin, difficult preservation of astaxanthin, etc. Effects of fish body color, increased growth rate and survival rate

Active Publication Date: 2021-03-23
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more astaxanthin is added to the feed to improve the body color of clownfish, but astaxanthin is expensive, the market price of 1 kg is as high as 1,600 yuan, and astaxanthin is not easy to preserve, so it is not suitable for long-term addition in feed

Method used

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  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example provides the isolation and identification of Bacillus licheniformis XCY26

[0032] 1. Isolation of Bacillus licheniformis XCY26

[0033] S1. Source of strain

[0034] The gut of Amphiprion ocellaris is darker in color.

[0035] S2. Isolation of strains

[0036] The body surface of Gongzi clownfish was wiped and disinfected with 75% alcohol, the intestinal tract was dissected aseptically, and the intestinal tissue was fully mixed with 1mL of 0.85% sterile saline. Normal saline was diluted 10-fold to obtain 10 -1 -10 -6 100 μL of each dilution was spread on 2216E, TCBS, MRS solid medium plates, and each dilution was done in 3 parallels, and placed in a 30°C incubator for 24 hours.

[0037] Select a plate with an appropriate dilution of growth density to observe the colony morphology, and conduct preliminary inspections according to the colony size, gloss, texture, edge features, surface features, bulge shape, transparency, colony and medium color, growth...

Embodiment 2

[0067] The present embodiment provides the preparation of bacillus licheniformis XCY26 bacterial agent

[0068] S1. Preparation of primary seed culture medium

[0069] Use an inoculation loop to pick an appropriate amount of Bacillus licheniformis XCY26 cells preserved on the slant and inoculate them into 6-10 250mL Erlenmeyer flasks containing 50mL of medium 1 for cultivation. Among them, the composition of medium 1 is: peptone 8-12g, beef extract 4-6g, glucose 16-25g, sodium chloride 4-6g, distilled water 1000mL; culture conditions are: culture temperature 28-30 ℃; initial pH value 6.9 -7.2; the rotation speed of the shaker is 200-260rpm; the cultivation time is 20-24h; obtain 300-500mL of the primary seed culture solution.

[0070] S2. Preparation of secondary seed culture medium

[0071] According to the ratio of 2-4%, inoculate the primary seed culture solution into 20-30 2000mL Erlenmeyer flasks filled with 400mL medium 2. Among them, the composition of medium 2 is: p...

Embodiment 3

[0077] The present embodiment provides the application of Bacillus licheniformis XCY26 bacterial agent

[0078] The bacillus licheniformis XCY26 microbial agent prepared by embodiment 2 is added to the clownfish basal feed according to the ratio of 0.05%-1.0% (basic feed composition such as figure 2 shown), the content of Bacillus licheniformis XCY26 reached 5.0×10 per gram of basal feed 6 -1.0×10 8 CFU / g. Preferably, the content of XCY26 per gram of basal feed reaches 1.0×10 7 CFU / g, can significantly improve the body color of Gongzi clownfish (such as image 3 , Figure 4 shown), and can improve the growth rate and survival rate of the male clownfish (so Figure 5 shown).

[0079] S1. Test material

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Abstract

The invention discloses bacillus licheniformis and application thereof. The bacillus licheniformis is specifically bacillus licheniformis XCY26, and the preservation number of the bacillus licheniformis XCY26 in the China General Microbiological Culture Collection Center is CGMCC No. 18463. The bacillus licheniformis XCY26 with CGMCC No.18463 can be used for remarkably improving the body color ofclownfish and increasing the growth speed and survival rate of the clownfish. Meanwhile, a bacillus licheniformis inoculant can replace astaxanthin, is low in cost, convenient to use and easy to store, and is a green and safe aquatic feed additive.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus licheniformis XCY26 and its application. Background technique [0002] Seawater ornamental fish were once only the pets of high-end aquarium players due to their high investment and difficulty in raising them. But along with the improvement of people's living standard and the maturation of relevant technology, seawater ornamental fish has gradually come into common people's home. Clownfish is one of the best varieties of seawater ornamental fish, and its price is not high, so it has gradually become the preferred species of seawater ornamental fish. [0003] Clownfish belong to the family Pomacentridae and the subfamily Amphiprioninae, which can be divided into the genera Amphiprion and Premna. They are mainly distributed in the reef waters at low latitudes in the western Pacific Ocean, swimming among coral reefs. Under natural conditions, clownfish live in s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/10
CPCC12N1/20A23K10/18A23K50/80Y02A40/81
Inventor 孙云章杨红玲魏成业汪攀杨伟
Owner JIMEI UNIV
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