Application of Wheat tafbx113 Gene in Regulating Grain Thickness Development

A wheat grain and wheat technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of unclear biological function and molecular mechanism of wheat grain thickness, and achieve the effect of increasing grain thickness

Active Publication Date: 2022-03-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relationship between the TaFBX113 gene and grain thickness has not been reported, and the biological function and molecular mechanism of the gene in affecting the grain thickness of wheat are still unclear

Method used

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  • Application of Wheat tafbx113 Gene in Regulating Grain Thickness Development
  • Application of Wheat tafbx113 Gene in Regulating Grain Thickness Development
  • Application of Wheat tafbx113 Gene in Regulating Grain Thickness Development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Genotyping of TaFBX113-B gene in core germplasm materials

[0041] 1. Select wheat micro-core germplasm materials (105 bred varieties and 157 local varieties) distributed in different wheat regions in my country as excavation materials for polymorphic SNP sites.

[0042]2. Exon capture obtained the SNP of the microcore germplasm material, and used the Roche exon capture kit (SeqCapPure Capture Bead Kit, 06977952001, Roche) to perform library construction and sequencing on the DNA of 262 materials. For specific methods, refer to the kit instruction manual .

[0043] 3. The whole genome sequence comparison was carried out by BWA software, the variation detection and genotyping were carried out by GATK software, and the genotype of the gene in 262 materials was obtained.

Embodiment 2

[0044] Example 2 Association analysis of TaFBX113-B gene SNP loci and grain thickness traits

[0045] Using the grain thickness phenotype data of 262 micro-core germplasm materials for three years and three points (in Luoyang, Henan Province in 2002, in Luoyang, Henan Province in 2005, and in Beijing in 2006), association analysis was carried out. The correlation results between TaFBX113-B gene and grain thickness are shown in Table 1. It can be seen that the SNP loci of TaFBX113-B gene are significantly correlated with the grain thickness traits of wheat materials.

[0046] Table 1 SNP sites significantly associated with grain thickness

[0047]

Embodiment 3

[0048] Example 3 The sequence of the wheat TaFBX113-B gene

[0049] According to the TraesCS3B02G145100 gene number, compare it on the wheat genome database website, obtain its homologous gene number TraesCS3D02G127000, and obtain the gene sequence of the TaFBX113-B gene on the D homologous chromosome, as shown in SEQ ID NO.4, the gene is located at the third Chromosomal homology group.

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Abstract

The invention relates to the application of wheat TaFBX113 gene in regulating grain thickness development, and belongs to the field of crop molecular biology. The invention provides the application of the wheat TaFBX113 protein and its coding gene or the suppressor of the TaFBX113 protein coding gene in regulating the grain thickness of plants. The present invention confirms in the EMS mutagenesis library that the grain thickness of the wheat mutant strain whose translation of TaFBX113 gene is prematurely terminated is significantly increased. The TaFBX113 gene provided by the invention has the function of regulating the development of the grain thickness of the plant, and plays an important role in improving and improving germplasm resources of crops such as wheat.

Description

technical field [0001] The invention relates to the fields of crop molecular biology and molecular breeding, and more specifically relates to the application of wheat TaFBX113 gene in regulating grain thickness. Background technique [0002] Wheat is an important food crop in the world, and it is also the staple food of more than 1 / 3 of the world's population. In recent years, my country has become the world's largest wheat producer and consumer, so the production of wheat is closely related to the country's food security. With the increase of population, the reduction of arable land and the increase of production cost, how to increase wheat production to ensure food security has become an urgent problem to be solved. Wheat grain thickness, as a component of 1,000-grain weight, has an important impact on the formation of yield. Studying the genetic law of grain thickness development is helpful to understand the mechanism of yield formation, and it is helpful for the use of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/156C12Q2600/13
Inventor 耿帅锋廖如意李爱丽毛龙
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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