High-stability homocysteine determination kit and preparation method thereof

Abstract

The invention relates to the technical field of medical diagnosis, and discloses a high-stability homocysteine determination kit and a preparation method thereof. The kit consists of a reagent R1 anda reagent R2. The reagent R1 is composed of a reagent obtained after BSA and glucose are heated and incubated, a buffer solution, TCEP, serine, NADH, TWEEN-20, glucose dehydrogenase, carboxyl microspheres and a preservative. The reagent R2 is prepared from a buffer solution, CBL, CBS, pyridoxal phosphate, LDH, a dissociation agent and a preservative. Glucose is fixed in a heat treatment mode of glucose and BSA, and meanwhile, serine and NADH are effectively protected through the fixed operation of physical adsorption of serine by carboxyl microspheres. The whole kit can show better performancein the aspects of 8-DEG C bottle opening stability, 37-DEG C thermal stability and 4-DEG C real-time stability at the same time, and is superior to similar products in the market.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a highly stable homocysteine ​​assay kit and a preparation method thereof. Background technique [0002] Homocysteine ​​(HCY), also known as homocysteine, homocysteine ​​or homocysteine, referred to as blood, is an essential amino acid for human nutrition (needed by the body but cannot be synthesized by itself) An important intermediate product in the metabolism of methionine. When the human body ingests protein from external food, the protein is decomposed into methionine under the action of hydrolytic enzymes in the body, and methionine will synthesize S-adenosyl homocysteine ​​(SAH) with a highly activated methyl group. Further removal of adenosine from SAH produces homocysteine. [0003] Homocysteine ​​mainly exists in cells, normal people will produce 15-20μmol / L homocysteine ​​every day, most of these homocysteine ​​will be catabolized in cells, of which about 1...

AI Technical Summary

Problems solved by technology

The cost of reagents for hydrolase cycle enzymatic method is high, and its detection sensitivity, ability to resist interfering substances, linearity and stability are easily affected

For most commercially available cystathionine cycle enzyme reagents, there are also common stability problems, including 2-8°C bottle opening stability, 37°C thermal stability and 4°C real-time stability

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