A method for rapid cultivation of nitrogen-efficient transgenic maize inbred lines

A technology of transgenic corn and breeding method, which is applied in the field of rapid breeding of nitrogen-efficient transgenic corn inbred lines, can solve the problems of time-consuming and labor-intensive, and achieve the effects of reducing workload, shortening the breeding cycle, and speeding up the breeding process

Active Publication Date: 2022-03-01
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is: how to solve the time-consuming and labor-intensive problem of returning the existing nitrogen efficient use transgenic receptor to the backbone line to be consistent with the genetic background

Method used

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  • A method for rapid cultivation of nitrogen-efficient transgenic maize inbred lines
  • A method for rapid cultivation of nitrogen-efficient transgenic maize inbred lines
  • A method for rapid cultivation of nitrogen-efficient transgenic maize inbred lines

Examples

Experimental program
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Effect test

Embodiment 1

[0082] 1. Hybridization of nitrogen-efficient transgenic transformant materials with recurrent parents (field planting)

[0083] Planting genetically stable nitrogen-efficient transgenic transformant materials (A1 to An) and reincarnated parents (B), using nitrogen-efficient transgenic transformant materials (A1 to An) as male parent, and recurrent parent (B) as female parent Hybridization was carried out to ensure that the transgenic transformant materials (A1 to An) with high nitrogen utilization efficiency were respectively obtained from the recurrent parent (B) to obtain F 1 There are more than 10 ears of hybrid fruit. A1 to An were the ears pollinated by the male parent, and 5 ears were respectively selected and reserved for seed (selection criteria: increased growth and yield of plants, typical ears, large number of grains in ears, and full grains).

[0084] Investigate and record the main agronomic and economic traits (such as: growth period, leaf sheath color, plant h...

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Abstract

The invention discloses a method for rapidly cultivating transgenic corn inbred lines with high nitrogen utilization efficiency. Under the condition of nitrogen stress in a nutrient pool, the nitrogen utilization efficiency of the first generation of hybrids is evaluated, and the transformants with the best nitrogen utilization efficiency and their hybrid singles are selected. Targeted backcross breeding and inbred line cultivation, through the pre-assessment of the efficient nitrogen utilization efficiency of the transformants, low-efficiency transformant materials and their backcross offspring were eliminated, which greatly reduced the workload and also The planting scale of the selected transformant material can be expanded to facilitate subsequent selection. "Nitrogen stress + large population + phenotype-genotype association selection" is used to assign values ​​according to the similarity between a certain trait and the recurrent parent traits, and assign and accumulate multiple traits, and perform phenotype-genotype on a single plant according to the score. Type association selection, homozygous selection of gene loci after comprehensive identification of 3-4 consecutive phenotypes, and backcross offspring with a background recovery rate of more than 99% can be obtained 2 years in advance.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to a method for rapidly cultivating transgenic maize inbred lines with high nitrogen utilization. Background technique [0002] Maize is a high-yielding crop and occupies an important strategic position in my country's and even the world's grain production. However, in recent years, in order to ensure the high and stable yield of corn, my country has used a large amount of chemical fertilizers in corn production, which has caused ecological and environmental problems such as soil compaction and acidification, eutrophication of water bodies, and increased acid rain. Improving the nitrogen use efficiency of corn can effectively reduce the amount of fertilizer application, reduce environmental pollution, and reduce production costs. The existing nitrogen-efficient maize germplasm resources in my country are extremely scarce, and the cultivation of new nitrogen-efficient tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 杨麟何文铸朱永卉何远远
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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