Bacillus subtilis Y2 strain and preparation method for preparing antagonistic Korla pear blackhead disease inhibitor by using bacillus subtilis Y2 strain

Abstract

The invention discloses a strain of bacillus subtilis Y2 and application thereof to prevention and treatment of the Korla pear blackhead disease. The Y2 strain is collected in China Center for Type Culture Collection in Wuhan University in China on October 30, 2018, and the collection number is CCTCC NO: M2018678. In plate antagonistic experiments, it is found that the Y2 strain has the activity of inhibiting the main pathogenic bacteria alternaria brassicae XL2 of the Korla pear blackhead disease, and the hypha growth inhibition rate reaches 42.74%; and a sterile filtrate of the Y2 strain also has very high bacteriostatic activity. Inoculation experiments on Korla pears prove that the Y2 strain can inhibit infection and colonization of pathogenic bacteria of the Korla pear blackhead disease. Therefore, the bacillus subtilis Y2 can be applied to prevention and treatment of the Korla pear blackhead disease as a biocontrol bacterium.

Description

technical field [0001] The invention relates to a bacillus subtilis (Bacillus subtilis Y2) and a preparation method of using the new strain to prepare an inhibitor against Korla fragrant pear blackhead, belonging to the field of microorganism technology. Background technique [0002] Korla fragrant pear is a traditional dominant pear variety with Xinjiang characteristics. It is a pear variety with a high market share in my country and a relatively high sales price among domestic pears. It is also one of the main export pear varieties in my country. However, the quality of Korla fragrant pears declines during postharvest storage, and they are prone to various diseases. Blackhead disease is a newly discovered postharvest disease of Korla fragrant pears in recent years. Alternaria brassicicola XL2. Pathogens invaded the pear fruit through the natural orifice, resulting in a black honeycomb mold layer in the pulp, and with the prolongation of storage time, the brown mold layer ...

AI Technical Summary

Problems solved by technology

Although a variety of Bacillus subtilis has been developed for the preparation of microbial fungicides, due to the diversity of Bacillus subtilis strains, the antibacterial spectrum, antibacterial activity and antibacterial characteristics of different strains of Bacillus subtilis in different regions are quite different, so Screening highly effective Bacillus subtilis biocontrol agents with different targets is still a research hotspot at present and in the future

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