Cell preserving fluid, preparation method thereof and cell preserving method
A preservation method and preservation solution technology, applied in the preservation, application, animal husbandry, etc. of human or animal bodies, can solve the requirements that it is difficult to ensure the number and quality of effective cells, the epithelial cells cannot be precipitated, and mucus substances cannot be dissolved, etc. problems, to achieve the effect of preserving multiple cell types, protecting the working environment and staff health, and preventing cell aggregation
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Embodiment 1-3
[0053] The formula of the cell preservation solution provided by Examples 1-3 is shown in Table 1 below:
[0054] Table 1
[0055] Example 1 Example 2 Example 3 Ethanol (95% pure) 40% (v / v) 45% (v / v) 50% (v / v) Tris 0.1% (w / v) 0.3% (w / v) 0.5% (w / v) EDTA·2Na 0.01% (w / v) 0.02% (w / v) 0.03% (w / v) bromelain 0.01% (w / v) 0.05% (w / v) 0.04% (w / v) Tris(2-carboxyethyl)phosphine hydrochloride 0.01% (w / v) 0.03% (w / v) 0.05% (w / v) Diisobutylnaphthalenesulfonic acid 0.10% (w / v) 0.07% (w / v) 0.08% (w / v) BSA 0.08% (w / v) 0.07% (w / v) 0.06% (w / v) Trehalose 0.04% (w / v) 0.03% (w / v) 0.03% (w / v) proclin300 0.01% (w / v) 0.02% (w / v) 0.03% (w / v) Deionized water 59% (v / v) 53.5% (v / v) 58% (v / v) glacial acetic acid 1.0% (v / v) 1.5% (v / v) 2.0% (v / v) pH(25℃) 6.9 7.0 7.2
[0056] The method for preparing the cell preservation solution of Example 1 is as follows (prepar...
Embodiment 4
[0063] This embodiment provides the method of using the cell preservation solution provided in the above-mentioned embodiments 1-3, as follows:
[0064] 1. After sampling according to the standard sampling procedure, put the samples (such as vaginal and cervical exfoliated cell specimens, sputum specimens, vaginal and cervical bloody specimens, needle aspiration cell specimens, etc.) into the sample bottle filled with cell preservation solution, and tighten the preservation tube Cover inspection;
[0065] 2. Shake for 5-10 minutes, draw 2-3ml of cell suspension to the production chamber, and let it settle naturally for 10 minutes;
[0066] 3. Take out the slides for Papanicolaou staining process, and then detect and interpret the cell morphology under the microscope.
experiment example 1
[0068] Using the cell preservation solution provided in Example 1, the method of Example 4 was used to preserve the vaginal and cervical exfoliated cell specimens, sputum specimens, vaginal and cervical bloody specimens, and needle aspiration cell specimens, and then make slides for microscopic examination to observe the cell morphology. see results Figure 1-Figure 4 .
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