Cell preservation solution, preparation method thereof, and cell preservation method
A technology for preserving liquid and cells, applied in the preservation, application, animal husbandry, etc. of human or animal body, which can solve the problem of difficulty in ensuring the number and quality of effective cells, inability to precipitate epithelial cells, and inability to dissolve mucous substances. , to achieve the effect of preserving many cell types, protecting the working environment and staff health, and preventing cell aggregation
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Embodiment 1-3
[0053] The formula of the cell preservation solution provided by Examples 1-3 is shown in Table 1 below:
[0054] Table 1
[0055] Example 1 Example 2 Example 3 Ethanol (95% pure) 40% (v / v) 45% (v / v) 50% (v / v) Tris 0.1% (w / v) 0.3% (w / v) 0.5% (w / v) EDTA·2Na 0.01% (w / v) 0.02% (w / v) 0.03% (w / v) Bromelain 0.01% (w / v) 0.05% (w / v) 0.04% (w / v) Tris(2-carboxyethyl)phosphine hydrochloride 0.01% (w / v) 0.03% (w / v) 0.05% (w / v) Diisobutylnaphthalenesulfonic acid 0.10% (w / v) 0.07% (w / v) 0.08% (w / v) BSA 0.08% (w / v) 0.07% (w / v) 0.06% (w / v) Trehalose 0.04% (w / v) 0.03% (w / v) 0.03% (w / v) proclin300 0.01% (w / v) 0.02% (w / v) 0.03% (w / v) Deionized water 59% (v / v) 53.5% (v / v) 58% (v / v) glacial acetic acid 1.0% (v / v) 1.5% (v / v) 2.0% (v / v) pH(25℃) 6.9 7.0 7.2
[0056] The method for preparing the cell preservation solution of Example 1 is as follows (prepar...
Embodiment 4
[0063] This embodiment provides the method of using the cell preservation solution provided in the above-mentioned embodiments 1-3, as follows:
[0064] 1. After sampling according to the standard sampling procedure, put the samples (such as vaginal and cervical exfoliated cell specimens, sputum specimens, vaginal and cervical bloody specimens, needle aspiration cell specimens, etc.) into the sample bottle filled with cell preservation solution, and tighten the preservation tube Cover inspection;
[0065] 2. Shake for 5-10 minutes, draw 2-3ml of cell suspension to the production chamber, and let it settle naturally for 10 minutes;
[0066] 3. Take out the slides for Papanicolaou staining process, and then detect and interpret the cell morphology under the microscope.
experiment example 1
[0068] Using the cell preservation solution provided in Example 1, the method of Example 4 was used to preserve the vaginal and cervical exfoliated cell specimens, sputum specimens, vaginal and cervical bloody specimens, and needle aspiration cell specimens, and then make slides for microscopic examination to observe the cell morphology. see results Figure 1-Figure 4 .
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