Application of ergothioneine substances in preparation of medicines for preventing and treating cerebral ischemic stroke
A technology for ischemic stroke and ergothioneine, which can be used in drug combinations, pharmaceutical formulations, organic active ingredients and other directions, and can solve problems such as undiscovered ergothioneine
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Embodiment 1
[0021] Example 1: Effect of ergothioneine on LPS-induced microglial inflammatory response
[0022] The effect of ergothioneine substances on LPS-induced Influence of microglial inflammatory response. details as follows:
[0023] 1. Test method:
[0024] 1.1 Cell culture:
[0025] Microglial BV2 cells were inoculated in DMEM high-glucose medium containing 10% fetal bovine serum (containing 1% double antibody) at 37°C, 5% CO 2 Cultured in an incubator with saturated humidity, passaged every 2-3 days, when BV2 cells adhered to the wall and grew well, they were seeded in 24-well plates for subsequent experiments.
[0026] 1.2 Griess method was used to detect the NO content released by cells in each group:
[0027] Take the BV2 cells in the logarithmic growth phase, and use 1×10 5 Inoculate in a 24-well plate at a density of one per well (5 replicate wells per group), set up the control group (medium without ergothioneine and LPS), and the model group (medium containing only ...
Embodiment 2
[0039] Example 2: The protective effect of ergothioneine on neurons
[0040] With reference to the method of patent CN109908154A, the protective effect of ergothioneine substances on neurons is investigated, as follows:
[0041] 1. Test method:
[0042] 1.1 Establishment of the oxygen-glucose deprivation / reoxygenation (OGD / R) model of rat primary cortical neurons:
[0043] Sodium dithionite (Na 2 S 2 o 4 ) dry powder to make it fully dissolved, so that the final concentration is 20mM, with NaHCO 3 Adjust the pH to 7.2, which is the anoxic solution. The primary cortical neuron cells were cultured in a 24-well cell culture plate for 7-10 days, and the cells in good growth state were selected for experiments. The experiment included blank control group, model control group, ketorolac administration control group, ergothioneine administration group I, ergothioneine administration II group, ergothioneine administration III group, and ergothioneine hydrochloride administration g...
Embodiment 3
[0058] Example 3: The protective effect of ergothioneine on ischemia-reperfusion-induced cerebral infarction in rats
[0059] With reference to the method of patent CN109908154A, the protective effect of ergothioneine substances on ischemia-reperfusion-induced cerebral infarction in rats is investigated, as follows:
[0060] 1. Test method:
[0061] 1.1 Construction of cerebral infarction model:
[0062] The cerebral infarction model was established by blocking the middle cerebral artery of SD rats with suture and performing ischemia-reperfusion.
[0063] 1.2 Test grouping and treatment:
[0064] The successfully modeled SD rats were randomly divided into 4 groups: model group, positive drug group, ergothioneine group, and ergothioneine hydrochloride group; wherein, the positive drug group was injected with ginadol through the tail vein at a dose of 15 mg / kg, the ergothioneine group was injected with ergothioneine through the tail vein, and the dose was 15 mg / kg; the ergot...
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