Potato whole-plant microorganism sample one-time preparation, separation and purification method

Abstract

The invention discloses a potato whole-plant microorganism sample one-time preparation, separation and purification method, which combines microorganism sampling and enlarged culture to establish a set of potato whole-plant microorganism separation and purification method, and can accurately and efficiently explore the flora structure and main functions of potato whole-plant microorganisms. Because different microorganisms need different culture media for growth, and all plant microorganisms cannot be expected to be separated by using one or more culture media, the invention provides a culture medium aiming at different potato leaves and root systems, so that a better culture effect can be achieved. According to the method, most types of samples involved in current potato microbiome research are separated, collected and extracted at a time, the samples comprise seven types including phyllosphere, stem girth, root zone, rhizosphere, root surface, root interior and tuber attachment, and the influence and interaction relation of external conditions such as different environments and the like on potato whole plant growth can be completely analyzed.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for one-time preparation and separation and purification of microbial samples of whole potato plants. Background technique [0002] In nature, plants coexist with various microorganisms. A large number of microorganisms attach to the surface and interior of plants and play an important role in plant growth and disease resistance. These microbial groups are collectively called the plant microbiome. The model plant Arabidopsis thaliana has shown great research and application value in the formation of bacterial populations and analysis of interactions. The aboveground part of a plant, called the phyllosphere, is a fixed, open, variable, circadian ecological environment in which its microbial habitat is exposed to rapid changes in ultraviolet light, temperature, moisture, and nutrients. The underground part of the plant is surrounded by soil, which is mainly affecte...

AI Technical Summary

Problems solved by technology

However, in the study of potato microbiome, there is no clear method to describe the sampling method of potato rhizosphere, phyllosphere, stem circumference, potato surface, etc. There are also few reports on the sequence of sampling in different parts, the definition of the root zone, rhizosphere, root surface, and root microbial areas, and their respective sampling methods.

Since there is no method for rapid acquisition and isolation and culture of potato whole plant microbial samples, the research progress of potato microbiome and culture omics is relatively lagging behind