Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for rapidly releasing intracellular DNA of gram-positive strain
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Gram-positive strains, which is applied in the field of rapid release of intracellular DNA of Gram-positive strains, can solve the problems of large labor investment, high detection cost, and large number of samples, and achieve the effect of reducing labor output
Pending Publication Date: 2021-04-20
林蠡 +1
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, this detection method is only effective for the detection of Gram-negative bacteria, and cannot directly detect Gram-positive bacteria. At present, the preparation of DNA templates involved in the identification of Gram-positive bacteria has become a Gram-positive method. An important resistance to the detection of Bacillus positive bacteria
[0013] Therefore, at present, the identification of bacterial species in the aquaculture industry is difficult to be popularized and promoted due to the characteristics of high detection frequency, long detection cycle, large number of samples, high detection cost, and large labor investment. There are no effective reagents and kits for wall breaking and DNA extraction of positive bacteria
Therefore, the traditional DNA extraction steps and identification methods are not suitable for the identification of Gram-positive bacteria
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0049] The method for rapidly releasing the intracellular DNA of Gram-positive bacterial species provided by the present embodiment comprises the following steps:
[0050] (1) Select 6 kinds of Bacillus (Gram-positive bacteria) probiotics products, use LB solid medium (peptone 10g / L, yeast extract 5g / L, NaCl10 g / L, agarpowder 15g / L) to carry out Flat plate coating, one flat plate for each sample;
[0051] (2) Use a parafilm to seal the plate, and place it in a 32°C incubator for 12 hours;
[0052] (3) Take out the solidculture plates of 6 samples, and pick a single colony for each sample and inoculate it on a plate containing 50 μL ddH 2 In the Eppendorf tube of O, the single colony is blown away, placed in the ice-water mixture for 3-5 minutes, and then placed in the freezing device to quickly reduce its temperature, and the ddH in the test tube 2 After O condenses into ice, let it stand still for 5-10 minutes, then quickly place it in a hot water bath, raise its temperat...
Embodiment 2
[0061] The difference from Example 1 is that the sample to be tested is aquaculture water.
[0062] ddH in the test tube to be prepared in step (3) 2 After the O condenses into ice, let it stand for 10 minutes, then quickly place it in a water bath at 100°C, raise the temperature to 100°C quickly, and let it stand for 20 minutes;
[0063] In step (4), after the solution in the test tube condenses into ice, let it stand still for 10 minutes, then quickly place it in a hot water bath, raise its temperature to 100° C., and let it stand still for 20 minutes.
[0064] In step (5), after the solution in the test tube condenses into ice, let it stand still for 10 minutes, then quickly place it in a hot water bath, make the temperature rise to 100° C. quickly, and let it stand still for 20 minutes.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for rapidly releasing intracellularDNA of a gram-positive strain. According to the method, a polymerasechain reaction (PCR) amplification template is quickly prepared from single colonies by three solutions including dd H2O, ET and ETS buffer and a physical variable temperature method, the steps of expanding culture and DNA extraction of the strain are directly skipped; and the method is quick and convenient, and the detection period, the detection cost and the labor output are greatly reduced.
Description
technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for rapidly releasing intracellularDNA of Gram-positive bacteria. Background technique [0002] Bacillus, a genus of bacteria, Gram-positive bacteria capable of forming spores (endospores). Bacillus can secrete a variety of extracellularproteases, hydrolytic enzymes, antimicrobial peptides, etc., which can effectively degrade organic matter in water and inhibit the proliferation of pathogenic bacteria in aquaculture water, and can also efficiently assimilate inorganic nitrogen and hydrogensulfide that are toxic to economic animals in aquaculture water and other substances to achieve the effect of water purification. At the same time, due to the characteristics of strong vitality, high tolerance and ability to produce spores, it provides favorable conditions for clinical application in industrial production and aquaculture. As a heterotrophic microbia...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More