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Application of DLK1/MEG3 locus in detection and treatment of growth hormone adenoma

A technology of growth hormone and gene loci, which is applied in the field of biomedicine, can solve the problem of high growth hormone and achieve the effect of accurate detection

Inactive Publication Date: 2021-04-30
BEIJING NEUROSURGICAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current treatment of growth hormone adenoma is mainly surgical treatment, but nearly 50% of patients still have high levels of growth hormone after surgery

Method used

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  • Application of DLK1/MEG3 locus in detection and treatment of growth hormone adenoma
  • Application of DLK1/MEG3 locus in detection and treatment of growth hormone adenoma
  • Application of DLK1/MEG3 locus in detection and treatment of growth hormone adenoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This example is used to illustrate that the DLK1 / MEG3 locus is highly expressed in somatotroph adenoma.

[0059] Using operations that meet the standards of the Medical Ethics Committee, the surgically resected tissues of 172 pituitary adenoma patients (age range 20 to 75 years old) who were treated at Beijing Tiantan Hospital between June 2018 and June 2019 were collected as the sample of this example. Pituitary adenoma samples were stored in liquid nitrogen, and each patient who collected samples obtained the consent of himself and his treating specialist before collecting samples, and had written certification materials.

[0060] According to the TIWI+C sequence of MR images and the results of transmission electron microscopy, according to the 2017 World Health Organization endocrine organ tumor classification standard, the 172 cases of pituitary adenoma samples in this example were classified, and the classification results showed that 45 cases of adrenocortical horm...

Embodiment 2

[0066] This example is used to illustrate that the expression level of the DLK1 / MEG3 locus is positively correlated with the level of growth hormone in serum, and negatively correlated with tumor volume.

[0067] According to the median value of the RNA-Seq TPM value of the DLK1 gene, the 31 somatotropin adenoma samples in Example 1 were divided into a high DLK1 group and a low DLK1 group, and the preoperative serum GH level of each sample corresponding to the patient and tumor volume for statistics, such as figure 2 , and then analyzed the correlation between the preoperative serum GH level and tumor volume of each patient and the DLK1 RNA-Seq TPM value, the analysis results are shown in image 3 and Figure 4 .

[0068] Depend on Figure 2-4 It can be seen that the expression level of the DLK1 / MEG3 locus is positively correlated with growth hormone levels in serum and negatively correlated with tumor volume.

Embodiment 3

[0070] This example is used to illustrate that the expression of the DLK1 / MEG3 locus can promote the differentiation of pituitary adenoma cells into somatotropin adenoma cells and inhibit the proliferation of tumor cells.

[0071] GH3 cell line (somatotroph adenoma) and MMQ cell line (prolactinoma) (ATCC, Manassas, VA, USA) were incubated at 37°C, 5% CO 2 F-12K medium (ATCC) supplemented with 2.5% fetal bovine serum and 10% horse serum. ATT20 cell line (adrenocorticotropic adenoma) (ATCC) at 37 ° C, 5% CO 2 cultured in a humidified incubator, DMEM (ATCC) medium supplemented with 10% fetal bovine serum.

[0072] The PIT1 level and DLK1 level in GH3 cell line, MMQ cell line and ATT20 cell line were measured respectively, and the test results were as follows Figure 5 shown by Figure 5 It can be seen that the GH3 cell line is a PIT1-positive cell line with high expression of DLK1, the MMQ cell line is a PIT1-positive cell line with low DLK1 expression, and the ATT20 cell line...

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Abstract

The invention relates to application of a DLK1 / MEG3 locus in detection and treatment of growth hormone adenoma, and quick and accurate detection of the growth hormone adenoma can be realized by identifying the DLK1 / MEG3 locus and / or an expression product of the DLK1 / MEG3 locus.

Description

technical field [0001] The disclosure relates to the technical field of biomedicine, and in particular, relates to the application of the DLK1 / MEG3 gene locus in the detection and treatment of growth hormone adenoma. Background technique [0002] Somatotropin adenomas are common functional adenomas that secrete excess growth hormone and insulin-like growth factor and cause acromegaly and gigantism. The current treatment method for growth hormone adenoma is mainly surgical treatment, but nearly 50% of patients still have high levels of growth hormone after surgical treatment. [0003] Rapid and accurate detection of growth hormone adenoma and timely and early detection of growth hormone adenoma are of great significance for its clinical treatment. Contents of the invention [0004] The purpose of the present disclosure is to provide the application of DLK1 / MEG3 locus in the detection and treatment of somatotroph adenoma. [0005] In order to achieve the above purpose, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12M1/34C12M1/00G01N33/574A61K45/00A61P35/00
CPCC12Q1/6886G01N33/57484A61K45/00A61P35/00C12Q2600/158
Inventor 高华陈一元张亚卓
Owner BEIJING NEUROSURGICAL INST
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