Kit for immunoblotting experiment by utilizing antibody and application of kit

Abstract

The invention discloses a kit for an immunoblotting experiment by utilizing an antibody and application of the kit, and belongs to the field of molecular biology. The invention aims to solve the problems that the immunoblotting reaction is difficult to operate and a great amount of time is consumed for searching and comparing reagents. The kit comprises an RIPA lysis solution, a PMSF, a BCA protein concentration determination reagent, an ECL luminescence detection solution, a pre-dyed protein Marker, Goat Anti-Rabbit IgGHRP, 10g of skim milk powder, a 10*PBS buffer solution, a 5*SDS-PAGE sample loading buffer solution, an electrophoresis buffer solution, a transfer buffer solution, a 10*TBST buffer solution and a PVDF film. The kit for the immunoblotting experiment is high in detection sensitivity, can be verified for multiple times, and is objective and accurate in experiment result.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit for immunoblotting experiment using antibodies and application thereof. Background technique [0002] Western is currently the most commonly used method for detecting proteins with antibodies in laboratories. However, there is currently a problem that the overall experimental operation of Western (immunoblotting) is difficult. The reason is that all required reagent consumables need to be purchased separately, and it takes a lot of time to find and compare reagents; all required reagents need to be prepared separately, adjusted in proportion, and verified for availability; many reagents cannot be used up at one time, resulting in waste; when optimizing experimental conditions, Considering that the difference is large, it is easy to cause inaccurate results. Contents of the invention [0003] The purpose of the present invention is to solve the problems of di...

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of difficult operation of western blotting reaction and the time-consuming problem of searching and comparing reagents. The present invention provides a kit for using antibodies to do western blot experiments. The kit includes: 5mL RIPA lysate, 50μL PMSF, BCA protein concentration determination reagent, 10mL ECL luminescence detection solution, 20uL pre-stained protein marker, 15μL Goat Anti-Rabbit IgG-HRP, 10g skimmed milk powder, 80mL 10×PBS buffer, 2mL 5×SDS-PAGE loading buffer solution, 200mL electrophoresis buffer, 150mL transfer buffer, 200mL 10×TBST buffer and 3 PVDF membranes

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