Prebiotic composition, composition and use thereof
A technology of composition and prebiotics, applied in the direction of drug combination, application, food science, etc., can solve problems such as unpublished demonstration results, and achieve the effect of preventing or treating diarrhea
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Embodiment 1
[0079] Bifidobacterium animalis BB12 strain was provided by Chr. Hansen.
[0080] (1) Put the BB12 strain into de Man Rogosa Sharpe (MRS) broth (Difco, Detroit, MD) supplemented with 0.5g / L L-cysteine and cultivate it in an anaerobic environment at 37°C for 24h , to obtain cultures. The culture was then passaged on semi-synthetic MRS medium (sMRS) supplemented with 1% (w / v) filter-sterilized glucose as the sole carbohydrate source + 0.5 g / L of L-cysteine Twice; after the second passage, the resulting culture was used as an inoculum for later use. Among them, various carbon source mother liquors were sterilized by filtration with a filter with a pore size of 0.22 microns and used at a final concentration of 1%.
[0081](2) Set galacto-oligosaccharide (GOS) group, isomerized lactose (LOS) group, GOS / LOS (weight ratio 1:1) group, GOS / LOS (weight ratio 4:1) group and GOS / LOS ( Weight ratio 9:1) group. Each group was inoculated with BB12 in MRS+0.05% cysteine medium accordi...
Embodiment 2
[0084] (1) With step (1) in embodiment 1.
[0085] (2) Set fructooligosaccharide (FOS) group, isomerized lactose (LOS) group, FOS / LOS (weight ratio 1:1) group, FOS / LOS (weight ratio 4:1) group and FOS / LOS (weight ratio than 9:1) group. Each group was inoculated with BB12 in MRS+0.05% cysteine medium according to the OD600 reading of 0.1, and added 1% fructo-oligosaccharide (FOS), 1% isomerized lactose (LOS), and 1% FOS / LOS respectively (weight ratio 1:1) composition, 1% FOS / LOS (weight ratio 4:1) composition and 1% FOS / LOS (weight ratio 9:1) composition, that is, BB12 bacteria and probiotics in each group Ratio of primary substances (FOS, LOS, FOS and LOS in a weight ratio of 1:1, FOS and LOS in a weight ratio of 4:1, or FOS and LOS in a weight ratio of 9:1) is 10 10 : 1CFU / g, the temperature of cultivation is 35 ℃~38 ℃, after cultivating 15 hours, utilize high-performance liquid chromatography (HPLC) to measure the OD600 in the above-mentioned each group culture medium, t...
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