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Prebiotic composition, composition and use thereof

A technology of composition and prebiotics, applied in the direction of drug combination, application, food science, etc., can solve problems such as unpublished demonstration results, and achieve the effect of preventing or treating diarrhea

Active Publication Date: 2021-05-04
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ISAPP (International Scientific Association for Probiotics and Prebiotics) organized top experts to demonstrate a definition of synbiotics in May 2019, but the results of the demonstration have not yet been released

Method used

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  • Prebiotic composition, composition and use thereof
  • Prebiotic composition, composition and use thereof
  • Prebiotic composition, composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Bifidobacterium animalis BB12 strain was provided by Chr. Hansen.

[0080] (1) Put the BB12 strain into de Man Rogosa Sharpe (MRS) broth (Difco, Detroit, MD) supplemented with 0.5g / L L-cysteine ​​and cultivate it in an anaerobic environment at 37°C for 24h , to obtain cultures. The culture was then passaged on semi-synthetic MRS medium (sMRS) supplemented with 1% (w / v) filter-sterilized glucose as the sole carbohydrate source + 0.5 g / L of L-cysteine Twice; after the second passage, the resulting culture was used as an inoculum for later use. Among them, various carbon source mother liquors were sterilized by filtration with a filter with a pore size of 0.22 microns and used at a final concentration of 1%.

[0081](2) Set galacto-oligosaccharide (GOS) group, isomerized lactose (LOS) group, GOS / LOS (weight ratio 1:1) group, GOS / LOS (weight ratio 4:1) group and GOS / LOS ( Weight ratio 9:1) group. Each group was inoculated with BB12 in MRS+0.05% cysteine ​​medium accordi...

Embodiment 2

[0084] (1) With step (1) in embodiment 1.

[0085] (2) Set fructooligosaccharide (FOS) group, isomerized lactose (LOS) group, FOS / LOS (weight ratio 1:1) group, FOS / LOS (weight ratio 4:1) group and FOS / LOS (weight ratio than 9:1) group. Each group was inoculated with BB12 in MRS+0.05% cysteine ​​medium according to the OD600 reading of 0.1, and added 1% fructo-oligosaccharide (FOS), 1% isomerized lactose (LOS), and 1% FOS / LOS respectively (weight ratio 1:1) composition, 1% FOS / LOS (weight ratio 4:1) composition and 1% FOS / LOS (weight ratio 9:1) composition, that is, BB12 bacteria and probiotics in each group Ratio of primary substances (FOS, LOS, FOS and LOS in a weight ratio of 1:1, FOS and LOS in a weight ratio of 4:1, or FOS and LOS in a weight ratio of 9:1) is 10 10 : 1CFU / g, the temperature of cultivation is 35 ℃~38 ℃, after cultivating 15 hours, utilize high-performance liquid chromatography (HPLC) to measure the OD600 in the above-mentioned each group culture medium, t...

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PUM

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Abstract

The invention belongs to the technical field of foods or medicines and particularly relates to a prebiotic composition. The prebiotic composition comprises the following ingredients in parts by weight: 0.5-18 parts of oligosaccharides and 1 part of isomerized lactose, wherein the oligosaccharides comprise at least one selected from galacto-oligosaccharides, fructo-oligosaccharides and raffinoses. The invention further relates to a (synbiotic) composition containing the prebiotic composition and bifidobacterium animalis and application of the prebiotic composition and the composition. The prebiotic composition disclosed by the invention can be used for remarkably promoting propagation of BB12; and the synbiotic composition disclosed by the invention can be used for preventing or treating diarrhea, antibiotics-induced intestinal canal flora disorder, ferrum-supplement-induced intestinal canal flora disorder or respiratory tract infection diseases.

Description

technical field [0001] The invention belongs to the technical field of food or medicine, and specifically relates to a prebiotic composition, a composition containing the prebiotic composition and animal bifidobacterium, and also relates to the prebiotic composition and the food or Medicinal use. Background technique [0002] The World Food and Agriculture Organization (FAO) and the World Health Organization (WHO) define probiotics as: live bacteria that can exert an effective effect on the health of the eater through ingestion of an appropriate amount. Among the many probiotics, bifidobacteria have been receiving much attention. A large number of studies have pointed out that bifidobacteria are closely related to people's health and diseases throughout their lives, such as figure 1 shown [1] : In the early life, bifidobacteria account for about 60% to 70% of the intestinal bacteria in the human body. The lack of bifidobacteria will lead to necrotizing enterocolitis, alle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21A23L33/135A23C9/16A23C9/123A61K35/745A61P1/12A61P1/00A61P11/00A61P31/00A61K31/702A61K31/715A61K31/7016A61K31/733
CPCA23L33/21A23L33/135A23C9/16A23C9/1234A61K35/745A61P1/12A61P1/00A61P11/00A61P31/00A61K31/702A61K31/715A61K31/7016A61K31/733A23V2002/00A23V2400/515A23V2200/3202A23V2200/314A23V2250/284A61K2300/00
Inventor 石羽杰刘彪
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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