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Klebsiella oxytoca as well as microbial agent and application thereof

A Klebsiella and microbial bacterial agent technology, applied in the field of microorganisms, can solve the problems of less research on erythromycin, achieve good development and application prospects, and achieve the effect of strong degradation ability

Active Publication Date: 2021-05-04
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few studies on microbial degradation of erythromycin at home and abroad

Method used

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  • Klebsiella oxytoca as well as microbial agent and application thereof
  • Klebsiella oxytoca as well as microbial agent and application thereof
  • Klebsiella oxytoca as well as microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and Identification of Tylosin Degrading Bacteria

[0045] 1. Domestication, isolation and purification of tylosin-degrading bacteria

[0046] Add 100ml of inorganic salt medium to a 250ml Erlenmeyer flask for use after sterilization, add 10g of soil, and add 50mg·L of tylosin -1 , yeast extract 0.2g, glucose 0.1g, 30℃, 180r·min -1 Add 100 mg·L after shaking culture for 3 days -1 Tylosin, 30°C, 180r·min -1 Shake culture for 3 days; transfer to conical flask containing 100ml inorganic salt liquid medium with 10% inoculum, add tylosin 100mg·L -1 , yeast extract 0.1g, glucose 0.05g, 30℃, 180r·min -1 Shake culture for 3 days; add mg·L -1 Tylosin, 30°C, 180r·min -1 Shaking culture for 3 days; after four rounds of acclimatization, the bacterial solution was -1 On the LB solid medium of tylosin, put it in a constant temperature incubator at 30°C and incubate in the dark for 24 hours, pick a single colony, and transfer it to a new medium containing 100...

Embodiment 2

[0058] Degradation characteristics of embodiment 2 Klebsiella oxytoca TYL-T1 (Klebsiella oxytoca TYL-T1)

[0059] a. Determination of growth curve and degradation curve

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Abstract

The invention relates to klebsiella oxytoca, in particular to klebsiella oxytoca TYL-T1, which is deposited in the China Center for Type Culture Collection (CCTCC) with a deposit number of CCTCC NO: M2021098. The invention also provides a microbial agent containing the klebsiella oxytoca and a preparation method of the microbial agent, and provides an application of the microbial agent in degradation of tylosin. The klebsiella oxytoca TYL-T1 has extremely high degradation capacity on tylosin, has a degradation rate on 100 mg / L tylosin up to 80.86% within 36 hours, and the highest degradation rate of 99.83% after 72 hours, can reach a degradation rate of more than 99% for 100mg / L tylosin after 72 hours, and has good development and application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Klebsiella oxytoca and its bacterial agent and application. Background technique [0002] Tylosin (TYL), also known as Tylosin and Tylosin, was a macrolide antibiotic obtained by American researchers from the culture medium of Streptomyces fradiae in 1959. It contains 4 components, wherein tylosin A accounts for 80% to 90% and has the highest antibacterial activity while other tylosin structures account for less. Tylosin A is the main active substance, followed by Tylosin B, and Tylosin D is not active. Tylosin is a white plate-like crystal, slightly soluble in water and alkaline. Its structure contains tartrate, phosphate, hydrochloride, sulfate and lactate, which are easily soluble in water. [0003] Tylosin is currently one of the macrolide veterinary antibiotics widely used in the aquaculture industry. The inhibitory effect of tylosin on Gram-positive bacteria, mycoplasma...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/22C02F101/38C02F101/34
CPCC12N1/20C02F3/34C02F2101/38C02F2101/34
Inventor 郑华宝张涛许双燕杨健赵卓群
Owner ZHEJIANG FORESTRY UNIVERSITY