A high spatial resolution surface sampling head and sampling method for mass spectrometry imaging based on microfluidic technology

Abstract

The invention relates to a mass spectrometry imaging high spatial resolution surface sampling head and a sampling method based on microfluidic technology. A mass spectrometry imaging high spatial resolution surface sampling head based on microfluidic technology, comprising a sampling head body, a sampling cavity is arranged at the bottom of the sampling head body, a first flow channel and a second flow channel are arranged inside the sampling head body, so the The first flow channel is used for injecting extraction solvent, the second flow channel is used for sample buffering and ejection; the first flow channel and the second flow channel both include a first interface and a second interface, the first flow channel and the second flow channel The first interfaces of the flow channels are respectively communicated with the sampling cavity, and the second interfaces of the first and second flow channels are respectively communicated with the outside of the sampling head body. Through the design of the sampling cavity and the flow channel, the invention has the characteristics of compact structure, at the same time improves the sampling spatial resolution and sampling effect, and has a flat design and a gas-liquid path interface, which can be easily connected with external robots such as robots. Combined with sports equipment.

Description

technical field [0001] The invention relates to a mass spectrometry imaging high spatial resolution surface sampling head and a sampling method based on microfluidic technology. Background technique [0002] Mass spectrometry is an analytical method based on mass-to-charge ratio detection, and is the only analytical method that can determine the structure of chemical molecules. [0003] Existing mass spectrometry technology adopts mass spectrometer pen to realize sample extraction, however, the existing mass spectrometer pen has the following disadvantages: [0004] 1) Large scale and low spatial resolution: The contact area between the mass spectrometer pen probe (sampling head) and the surface to be sampled is large, the diameter of the sampling area is 2-5mm, and the sampling area is significantly larger than 10mm 2 , so the spatial resolution is lower. Due to the limitation of the process, the above-mentioned invention cannot continue to improve the resolution greater ...

AI Technical Summary

Problems solved by technology

[0004] 1) Large scale and low spatial resolution: the contact area between the mass spectrometer probe (sampling head) and the surface to be sampled is relatively large, the diameter of the sampling area is 2-5mm, and the sampling area is obviously larger than 10mm 2 , so the spatial resolution is low

Due to the limitations of the technology, the aforementioned invention has a resolution greater than 1mm and cannot be further improved.

[0005] 2) Long tube transmission loss and pollution: The mass spectrometer probe (sampling head) and the sample collected by the aforementioned patent need to be transmitted to the mass spectrometer through a long tube. Long-distance transmission of the sample will cause residual loss and pollution, which will affect the analysis effect

[0006] 3) The sampling process and the analysis process are highly coupled: because the sample transfer of the mass spectrometer pen technology requires the mass spectrometer to provide vacuum suction and directly inject the sample, the sampling process and the analysis process are highly coupled, the system integration is difficult, and all samples are lost after analysis and cannot be saved. recheck

The aforementioned patent may combine the control of the peristaltic pump for sample delivery with the sample injection of the mass spectrometer, which is difficult to decouple

[0007] 4) Unable to integrate array automation: the current handheld sampling method and sampling head design of mass spectrometer pen technology are difficult to integrate with automation technology and equipment, and automatic sampling and analysis cannot be realized

Therefore, it is difficult to improve efficiency or realize remote operation

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