Response surface method optimized lamiophlomis rotata polysaccharide enzymolysis extraction process
An extraction process and response surface methodology technology, applied in the field of response surface methodology to optimize the enzymatic hydrolysis extraction process of unique polysaccharides, can solve the problems of low extraction rate, unable to exert maximum value, etc., and achieve the effect of convenient operation.
Inactive Publication Date: 2021-05-11
QINGDAO AGRI UNIV
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According to research reports, unique polysaccharides can be obtained by using water extraction and alcohol precipitation method, but the extraction rate of this extraction method is low, and unique Chinese medicine is a famous and precious traditional Chinese medicine, and its maximum value cannot be exerted
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Embodiment 1
[0030] The response surface method of this embodiment optimizes the unique polysaccharide enzymatic hydrolysis extraction process, including the following steps:
[0031] Step 1: Heat and reflux the Duyiwei powder twice with 80% ethanol, degrease, decolorize, remove oligosaccharides and small molecular impurities, and evaporate the solvent to obtain a processed sample;
[0032] Step 2: Weigh 3g of the pretreated sample, add an appropriate amount of distilled water, soak for 2 hours, control the single factor of enzyme amount, enzymolysis temperature, and Ph value, and then extract it in a water bath, collect the supernatant and concentrate it under reduced pressure to the original The volume is less than 20%, add absolute ethanol until the final concentration of ethanol is 80%, alcohol precipitation at 4°C for 24 hours, collect the precipitate after centrifugation, use the Sevag method to deproteinize, and use a dialysis bag with a molecular weight cut-off of 3500Da to dialyze ...
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The invention discloses a response surface method optimized lamiophlomis rotata polysaccharide enzymolysis extraction process. According to the process, the lamiophlomis rotata crude polysaccharide is extracted through enzymolysis, the enzyme dosage, the temperature, the extraction time and the extraction times are used as influence factors, the polysaccharide extraction rate is used as an investigation factor, and the optimal influence factor of the polysaccharide extraction rate is screened out through single factor investigation. By utilizing response surface design, the optimal extraction process is obtained by optimization: the temperature is 60 DEG C, the extraction time is 30 minutes, the enzyme-drug ratio is 2%, and the pH is 8. The optimal extraction process conditions of the lamiophlomis rotata polysaccharide are determined as follows: the extraction time is 60 minutes, the pH value is 8 and the extraction temperature is 60 DEG C through single factor investigation and RSM test. Results of the research show that the method for extracting the lamiophlomis rotata polysaccharide through enzymolysis is effective, convenient to operate, reliable and feasible, and the optimal extraction process conditions are that the extraction time is 60 minutes, the pH is 8, the extraction temperature is 60 DEG C, and the enzyme-drug ratio is 2%.
Description
technical field [0001] The invention relates to the technical field of unique polysaccharide extraction, in particular to the optimization of the unique polysaccharide enzymolysis extraction process by response surface method. Background technique [0002] The unique flavor of Tibetan medicine is used by ethnic minorities. It was first recorded in the masterpieces of Tibetan medicine "Four Medicines" and "Jingzhu Materia Medica". It has a history of 1,200 years. Its taste is sweet, slightly bitter, warm, and it is mainly used for joint bones and dried yellow water. It is used in modern clinics to treat bruises, bone pain, inflammation of spongy bone, etc. It has obvious analgesic and anti-inflammatory effects, and its curative effect is reliable. Domestic and foreign researches have reported that the unique herbal medicine has anti-oxidant, anti-inflammatory and analgesic pharmacological activities, but all attention is paid to the iridoids in the unique medicine, and there...
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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 候冉冉李雨婷邵湘玲王硕宇姜秩浩卢道冉李友英袁千丝
Owner QINGDAO AGRI UNIV