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Pyrite nano-enzyme, antitumor drug and application

An anti-tumor drug, pyrite technology, applied in the field of nanomaterials and tumor treatment, can solve very few problems

Active Publication Date: 2021-05-14
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such combined apoptosis-ferroptosis strategies are currently rare

Method used

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  • Pyrite nano-enzyme, antitumor drug and application
  • Pyrite nano-enzyme, antitumor drug and application
  • Pyrite nano-enzyme, antitumor drug and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Prepared as follows:

[0039] S1 Disperse 0.7g PVP in 30mL ethylene glycol solution, add 0.5g FeCl under continuous stirring 3 .6H 2 O, get A;

[0040] S2 Stir A vigorously, and add 3.6 g of NaAc while stirring to obtain B;

[0041] S3 Add 0.4g of S powder to B, ultrasonically react for 1h, and obtain C;

[0042] S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12 hours, and cool to room temperature after the reaction to obtain D;

[0043] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum freeze dryer Dry overnight.

Embodiment 2

[0045] Prepared as follows:

[0046] S1 Disperse 0.6g PVP in 30mL ethylene glycol solution, add 0.5g FeCl under continuous stirring 3 .6H 2 O, get A;

[0047] S2 Stir A vigorously, and add 3.6 g of NaAc while stirring to obtain B;

[0048] S3 Add 0.3g of S powder to B, ultrasonically react for 1h, and obtain C;

[0049] S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12 hours, and cool to room temperature after the reaction to obtain D;

[0050] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum freeze dryer Dry overnight.

Embodiment 3

[0052] Prepared as follows:

[0053] S1 Disperse 0.8g PVP in 30mL ethylene glycol solution, add 0.5g FeCl under continuous stirring 3 .6H 2 O, get A;

[0054] S2 Stir A vigorously, and add 3.6 g of NaAc while stirring to obtain B;

[0055] S3 Add 0.6g of S powder to B, ultrasonically react for 1 hour, and obtain C;

[0056] S4 Transfer C to a 40mL polytetrafluoroethylene-lined stainless steel autoclave, seal and keep it at 473K for a high temperature reaction for 12 hours, and cool to room temperature after the reaction to obtain D;

[0057] S5 Centrifuge D at 10,000 rpm for 10 minutes to collect a black precipitate, wash the collected black precipitate with chloroform to remove the residual S, then wash with absolute alcohol and ultrapure water to remove impurities, centrifuge and store in a vacuum freeze dryer Dry overnight.

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Abstract

The invention provides a pyrite nano-enzyme, an antitumor drug and application. The pyrite nano-enzyme comprises Fe < 2 + > and S 2< 2->, the atomic ratio of Fe to S is 1: 2, and the pyrite nano-enzyme presents a FeS2 crystal form. The pyrite nano enzyme has peroxidase activity (POD) and glutathione oxidase activity (GSH-OXD) at the same time; the catalytic efficiency (kcat / KM) of converting H2O2 into. OH under the active catalysis of POD is (8.5-9.5) * 10 < 8 > s <-1 > M <-1 >, and the catalytic efficiency (kcat / KM) of oxidizing GSH into GSSG and H2O2 under the active catalysis of GSH-OXD is (1-1.4) * 10 < 8 > s <-1 > M <-1 >. The POD and GSH-OXD activities of the pyrite nano-enzyme can form a cascade reaction to cause tumor cells to generate. OH and to deplete GSH in the cells, so that apoptosis and ferroptosis of the tumor cells occur; the killing effect has tumor cell specificity, and the killing effect on normal cells is very weak; the pyrite nano-enzyme has degradability, so that excellent safety is ensured; the pyrite nano-enzyme can exert POD and GSH-OXD activities in tumor cells in vivo, and can achieve an excellent treatment effect in apoptosis-resistant CT26 tumors containing KRAS mutations by inducing apoptosis and ferroptosis of tissues.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and tumor treatment, in particular to pyrite nanozymes, antitumor drugs and applications. Background technique [0002] Since Fe was first reported in 2007 3 o 4 Nanoparticles have peroxidase-like activity, and nanozymes, a class of nanomaterials with inherent enzymatic properties, have formed an emerging field. As a new generation of mimic enzymes, nanozymes are economical, stable, and easy to produce in large quantities. More importantly, nanozymes combine the catalytic activity of enzymes with the unique physical and chemical properties of nanomaterials, making it a multifunctional molecule. Different nanozymes have been widely used as substitutes for natural enzymes in molecular detection, immunoassay, antibacterial, and environmental governance. In particular, the ability of nanozymes to regulate reactive oxygen species (Reactive oxygen species, ROS) can be used in tumor therapy, suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/26A61K47/54A61K47/68A61P35/00B82Y5/00C01G49/12
CPCA61K33/26B82Y5/00A61P35/00A61K47/68A61K47/546A61K47/545C01G49/12C01P2002/72C01P2002/85C01P2004/03C01P2004/32C01P2004/62
Inventor 阎锡蕴高利增范克龙孟祥芹
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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