Primer pair composition, kit and method for distinguishing Yellow River carp from non-Yellow River carp carp
A technology for Yellow River carp and primer pairs, which is applied in the fields of biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc., can solve the damage to the reputation of the Yellow River carp, infringe on the legitimate rights and interests of consumers, and interfere with the normal operation of the Yellow River carp-related industries. and other problems, to achieve the effect of low cost, simple and convenient operation
Active Publication Date: 2022-05-17
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology
On the one hand, it infringes the legitimate rights and interests of consumers; on the other hand, it damages the reputation of the Yellow River carp and interferes with the normal operation of the related industries of the Yellow River carp.
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[0069] A method for distinguishing Yellow River carp from non-Yellow River carp Cyprinus, comprising:
[0070]1. Collect samples and extract genomic DNA
[0071] A total of 70 samples of the genus Carp from the Yellow River carp were collected from the Henan section of the main carp production area of the Yellow River; samples from non-Yellow River carp carps from the main carp production areas of Shandong, Liaoning and Heilongjiang were collected, including purse red carp, Furui carp, Songpu carp There are 230 mirror carps, Jian carps and high-yield carps in total.
[0072] Genomic DNA was extracted using a tissue DNA extraction kit (GeneJET, Thermo Scientific, USA) according to the instructions, and the site was carp back muscle tissue. The genomic DNA obtained after extraction was first measured with a spectrophotometer for the concentration and purity of the sample, then separated by gel agarose electrophoresis (electrophoresis voltage was 110V, time 45-50min), and the ...
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The application provides a primer pair composition, a kit and a method for distinguishing Yellow River carp from non-Yellow River carp Cyprinus, belonging to the technical field of molecular identification. The primer pair composition comprises the labeled primer pair of following nucleotide sequence: SEQ ID NO.1 and 2, NO.3 and 4, NO.5 and 6, NO.7 and 8, NO.9 and 10, NO.11 and 12, NO.13 and 14, NO.15 and 16, NO.17 and 18, NO.19 and 20, and NO.21 and 22. The kit includes the above-mentioned primer pair composition, and positive control primers whose nucleotide sequences are SEQ ID NO.23 and 24. Using the primer pair composition or the kit to perform PCR amplification on the genomic DNA of carp samples, and then determine the genus of Cyprinus according to the amplification conditions, the genus of Cyprinus can be accurately identified and distinguished from Yellow River carps and non-Yellow River carps, and the distinguishing method is simple and convenient. And the cost is low.
Description
technical field [0001] The present application relates to the technical field of molecular identification, in particular to a primer pair composition, a kit and a method for distinguishing Yellow River carp from non-Yellow River carp Cyprinus. Background technique [0002] The Yellow River carp is well-known at home and abroad for its long body, golden scales and red tail, and delicious meat; moreover, the Yellow River carp grows fast, has strong stress resistance and high nutritional value, so it is favored by farmers and consumers and has a broad market prospect. The market price of Yellow River carp is much higher than that of ordinary carp. Driven by economic interests, unscrupulous merchants sell counterfeit Yellow River carp from time to time. On the one hand, it infringes on the legal rights and interests of consumers; on the other hand, it damages the reputation of the Yellow River carp and interferes with the normal operation of the related industries of the Yellow...
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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125Y02A40/81
Inventor 杨曙明王彦云陈爱亮徐贞贞赵燕王济世刘晓夏
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS