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A strain of Pseudomonas monkeys for preventing and controlling Dactylodes and its fermentation broth and application

A technology of pseudomonas and fermentation broth, applied in the field of microorganisms, can solve the problems of affecting the quality and yield of sweet potato, environmental pollution, loss of sweet potato production and the like

Active Publication Date: 2022-07-12
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the sweet potato is infected, its skin turns brown, with cracks and bran cores, which greatly affects the quality and yield of sweet potato, and causes great losses to sweet potato production.
However, there are few effective agents on the market that can prevent and control stem nematodes, and they are mainly highly toxic chemicals, but they are easy to cause pollution to the environment
Therefore, biological control is an environmentally friendly, safe and effective development direction, but so far there is a lack of effective biological control strains

Method used

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  • A strain of Pseudomonas monkeys for preventing and controlling Dactylodes and its fermentation broth and application
  • A strain of Pseudomonas monkeys for preventing and controlling Dactylodes and its fermentation broth and application
  • A strain of Pseudomonas monkeys for preventing and controlling Dactylodes and its fermentation broth and application

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Embodiment 1

[0024] The embodiment of the present invention provides a strain of Pseudomonas monkey, which is obtained by screening through the following steps:

[0025] 1. Strain screening

[0026] After observation, it was found that some catalpa trees had serious root-knot nematode disease, and some catalpa trees had mild or no disease. Catalpa tree saplings with mild root-knot nematode disease were selected, and the root tissue of the catalpa tree was collected. The collected catalpa tree root tissue was washed with sterile water for sediment and surface attachments, and 0.02 g was weighed and put into an ultra-clean workbench to dry the water, soaked in 75% ethanol solution for 1 min, and rinsed with sterile water once. Soak in 3% sodium hypochlorite for 1 min, then rinse with sterile water 3 to 4 times, and apply the water from the last cleaning to the LB plate to check whether the disinfection is complete. After the completely sterilized samples were air-dried on the ultra-clean w...

Embodiment 2

[0078] The embodiment of the present invention provides a preparation method of the fermentation broth of Pseudomonas monkey NSJK strain:

[0079] The single colony of the Pseudomonas monkey NSJK strain obtained in Example 1 was streaked and cultured on the NA medium, cultured at 28°C for 24 hours, and then a single colony was picked and placed in the NB medium, 28°C, shaking at 200 r / min, Cultivated for 48h to prepare bacterial suspension. The obtained bacterial suspension was centrifuged at 8000 r / min for 5 min, and the supernatant was taken to obtain the fermentation broth of Pseudomonas monkey NSJK strain.

Embodiment 3

[0081] The embodiment of the present invention provides an investigation of the killing effect of the fermentation broth of Pseudomonas monkeys NSJK strain obtained in Example 2 on Stem nematodes.

[0082] First, pick a single colony of Pseudomonas monkeys NSJK strain on NA medium for streak culture, cultivate at 28°C for 24h, then pick a single colony and put it into NB medium, at 28°C, shake at 200 r / min, and culture for 48 hours. A bacterial suspension was prepared, and the viable count of Pseudomonas monkey NSJK in the bacterial suspension was 13×10 8 cfu / g. Pipette 5 μl of D. nematode suspension with a pipette, and microscopically examine, there are about 50 D. nematodes; suck 500 μl of sterile water to flush the D. nematode suspension into a 1.5 ml centrifuge tube, and examine again by microscopy to see if there is any residual D. nematodes on the slide. ; The experimental group was added with the supernatant of 500 μl, 8000r / min, centrifuged for 5 min of bacterial ferm...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a strain of Pseudomonas monkeys for preventing and treating Dactyloides nematodes and its fermentation broth and application. The Pseudomonas monkey has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on December 15, 2020, and its preservation number is CGMCC No. 21378; the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. No. The Pseudomonas monkeys can effectively prevent and control stem nematodes, and compared with 5% abamectin, the Pseudomonas monkeys has better control effect, stable subculture, and stable inheritance of nematicidal activity. The Pseudomonas monkey is safe for plant growth and harmless to the environment.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Pseudomonas monkeys for preventing and treating Dactyloides, and its fermentation broth and application. Background technique [0002] For many years, sweet potato stem nematode has been a serious disease in sweet potato growing areas. D. sweetpotato has strong adaptability to the environment and can survive for more than one year in a dry environment. D. nematode eggs, larvae and adults can overwinter together in sweet potato pieces or in fertilizer and soil. Stem nematodes are endoparasites with many routes of transmission and can be infected in seedbeds, fields and storage periods. After infection, the skin of sweet potato is brown, with cracks and bran cores, which greatly affects the quality and yield of sweet potato, and causes great losses to sweet potato production. However, currently there are few effective pesticides on the market that can control ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P5/00C12R1/38
CPCC12N1/20A01N63/27Y02A50/30
Inventor 闫红飞刘贺孟庆芳赵娜李令蕊邸宝房小力郝晓宇张文朝
Owner HEBEI AGRICULTURAL UNIV.
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