Compositions and methods for reducing atmospheric methane and nitrous oxide emissions
A composition, methane technology, applied in biochemical equipment and methods, microorganism-based methods, agricultural gas emission reduction, etc., which can solve problems such as increased nitrous oxide emissions
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[0129] Preparation of Microbial-Based Products
[0130] The present invention provides microbial-based products that reduce methane emissions from livestock production. A microorganism-based product of the invention is simply a fermentation medium comprising the microorganisms and / or microbial metabolites and / or any residual nutrients produced by the microorganisms. Fermented products are used directly without extraction or purification. Extraction and purification can be readily achieved, if desired, using standard extraction and / or purification methods or techniques described in the literature.
[0131] In one example, the yeast fermentation product designated "Star 3+" can be obtained by culturing the killing yeast, Wickhamiana anomalies, using a modified form of solid state fermentation. Cultures can be grown on surfaces with sufficient surface area for yeast to attach to and multiply on, such as rice, soybeans, chickpeas, pasta, oatmeal, or beans. For example, whole ye...
Embodiment 1
[0187] Example 1 - Production of Sophorolipids
[0188] Fermentation temperatures are typically between about 22-28°C, depending on the microorganisms and / or microbial growth by-products being cultured. For Candida globosa, a temperature of about 25°C is optimal.
[0189] The pH should be from about 2.0 to about 7.0, preferably from about 3.0 to about 6.5, depending on the microorganisms and / or microbial growth by-products being cultured. Additionally, to reduce the possibility of contamination, the cultivation process can be initiated at a first pH range and then adjusted to a second pH range above or below the first pH range.
[0190] Under these culture conditions, industrially useful production of biomass, biosurfactants and other metabolites is achieved after a minimum of 24 hours of fermentation. After the fermentation is complete, the growth by-products and / or remaining culture can be harvested from the reactor and used for a variety of industrial purposes.
[0191] ...
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