Preparation method and application of a kind of bacterial wall modified liposome carrying doxorubicin

Abstract

The invention discloses a preparation method and application of bacterial wall-modified liposome-loaded doxorubicin, which comprises dissolving soybean lecithin, cholesterol and doxorubicin in an organic solvent and rotary evaporation to obtain a dry lipid film ; The lipid film is hydrated, and the bacterial wall is added while hydrating to obtain DOX‑BW‑LP; the bacterial wall is the bacterial wall of Staphylococcus aureus. In the present invention, bacterial wall fusion liposomes are prepared into immunoliposomes, which act on human tongue squamous cell carcinoma cells, and it is found that the bacterial wall of Staphylococcus aureus prepared by the present invention has an immunoregulatory effect on macrophages, and induces macrophages to M1 polarization can enhance the recognition and phagocytosis of macrophages on tumor cells, and realize the functions of killing tumors and inhibiting tumor growth. The multiple roles of tumors provide new ideas for immunotherapy of oral cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of bacterial wall-modified liposome-loaded doxorubicin. Background technique [0002] Oral cavity cancer is one of the most common malignant tumors in the head and neck, and squamous cell carcinoma accounts for more than 90% of oropharyngeal malignant tumors. Oral squamous cell carcinoma (OSCC) has a high recurrence rate and is prone to metastasis. The 5-year survival rate of early stage patients is about 55%-60%, and the 5-year survival rate of advanced stage patients drops to 30%-40%. Unfortunately, 60% to 80% of cases are diagnosed at advanced stage, and it is estimated that about 145,328 patients die of oral squamous cell carcinoma worldwide every year. With the continuous improvement of diagnosis and treatment techniques, the survival rate of oral squamous cell carcinoma has been continuously improved, but it still faces...

AI Technical Summary

Problems solved by technology

Worst of all, tumors tend to recur and metastasize, resulting in poor quality of life for patients

Tumor immunotherapy is a means of mobilizing the body's own immune system to kill cancer cells and inhibit their proliferation through artificial intervention. It is the fourth major therapy for tumors after surgery, radiotherapy, and chemotherapy. It has great development potential, but tumor immunotherapy is still There are problems such as uncertain curative effect, narrow application range and many immune-related complications. Therefore, it is an important direction for current clinical research to find the optimal way of immunotherapy. Bacteria-mediated tumor therapy has been proved to be a promising strategy.

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