Electrochemical detection method for circulating miRNA in serum based on enzyme-free cascade circulating signal amplification

Abstract

The invention discloses an electrochemical detection method for circulating miRNA in serum based on enzyme-free cascade circulating signal amplification. The method mainly comprises the following steps of electrodepositing gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) to obtain a nanogold modified glassy carbon electrode AuNPs / GCE, designing a methylene blue labeled signal probe and a DNAzyme / MNAzyme shearing substrate chain, and synthesizing a dumbbell-shaped composite probe, designing a cascade cycle signal amplification method, and constructing an electrochemical sensing interface, and detecting circulating miRNA in serum. According to the electrochemical detection method for circulating miRNA in serum based on enzyme-free cascade circulating signal amplification, a simple, convenient and sensitive electrochemical sensing platform for circulating miRNA in serum is constructed by utilizing the ingenious design of a dumbbell-shaped composite probe and combining the high catalytic property, specificity and stability of DNAzyme / MNAzyme. The method has great potential of becoming a universal detection platform for different nucleic acid disease markers.

Description

technical field [0001] The invention relates to a DNA electrochemical sensor, in particular to an electrochemical detection method for circulating miRNA in serum based on enzyme-free cascade circulating signal amplification. Background technique [0002] MicroRNA (miRNA) is about 18-25 nucleotide bases, non-coding short RNA molecules, which play an important role in gene expression and regulation in most eukaryotes. Although miRNA molecules are prone to degradation, circulating miRNAs exhibit high stability in body fluids such as blood. Studies have shown that the expression of circulating miRNAs is associated with a variety of diseases, including tumor metastasis, diabetes, viral infection, and tissue damage. Therefore, circulating miRNAs can be used as minimally invasive biomarkers for the diagnosis of human diseases. [0003] However, due to the low abundance and sequence similarity of miRNAs, the detection of circulating miRNAs still faces great challenges, and it is u...

AI Technical Summary

Problems solved by technology

Although these methods provide useful sensing platforms for the detection of miRNAs, their complicated manipulations or limited sensitivity limit the possibility of their practical application

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