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3D bioprinting method based on nutrient flow channel

A 3D printing and 3D printer technology, applied in 3D object support structures, processing and manufacturing, manufacturing tools, etc., can solve problems such as unfavorable cell-carrying printing, inflexible printing methods, and inability to manufacture large-scale cell structures, and achieve guaranteed guarantees. Trueness and strength, high structural fidelity, effects that enhance print performance

Active Publication Date: 2022-08-02
苏州永沁泉智能设备有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned traditional scheme can obtain the printed object to a certain extent, the above-mentioned preparation method can improve the strength of the hydrogel structure, but it is not conducive to the printing of loaded cells, and the printing method is not flexible enough to manufacture large-scale cell structures with nutrient networks. body manufacturing

Method used

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  • 3D bioprinting method based on nutrient flow channel
  • 3D bioprinting method based on nutrient flow channel
  • 3D bioprinting method based on nutrient flow channel

Examples

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Embodiment 1

[0041] refer to figure 1 As shown, this embodiment provides a biological 3D printing method based on a nutrient flow channel, including the following steps:

[0042] S10, configure the sacrificial material and the matrix material respectively, load the configured sacrificial material and the matrix material into the multi-channel pipeline, and connect the multi-channel pipeline to the syringe pump system, so that the thrust of the syringe pump system matches the moving speed of the 3D printer, The material is pushed and extruded, and the syringe of the syringe pump system is loaded on the 3D printer (for example, the syringe of the syringe pump system can be loaded on the Z axis of the 3D printer).

[0043] The above-mentioned sacrificial material may include gelatin and other materials with good biocompatibility, printability, and easy removal. The above-mentioned matrix material may include GelMA (gelatin anhydride methacrylate) and other materials with good biocompatibili...

Embodiment 2

[0075]In this embodiment 2, taking gelatin as the sacrificial material and GelMA as the matrix material as an example, the device for realizing the above-mentioned nutrient flow channel-based biological 3D printing method may include:

[0076] The three-dimensional moving device of the motor module with controlled XYZ three coordinate axes, a set of syringe pump system with controllable flow rate, a set of refrigeration platform with real-time temperature control, and a multi-inlet nozzle.

[0077] The overall printing is embodied in the three-dimensional mobile device, which is the basic biological 3D printing device, which is composed of a motor module, a nozzle, and a base plate platform. The limit switch used by the motor is fixed on the module motor base on the motor side through two screws, and its function is to control the positioning and protect the terminal limit. Once the motor moves to the zero position, the limit switch is turned on to stop its movement, which can...

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Abstract

The invention relates to a biological 3D printing method based on a nutrient flow channel. The configured sacrificial material and matrix material are loaded into a multi-channel pipeline, the multi-channel pipeline is connected to a syringe pump system, and the syringe of the syringe pump system is loaded into the On the 3D printer; adjust the surface temperature of the cooling platform, turn on the light emitter, and extrude the multi-channel material. After the material can be stably discharged, turn on the printing parallel nozzle and start the printing according to the slicing path according to the organ file to be printed, and get the print. Model, irradiate and print the model to form a stable solid state structure, place the stable solid state structure on an oscillator, and dynamically cultivate the stable solid state structure in an incubator for a period of time to obtain a biological tissue structure with a nutrient network, which is By removing the sacrificial layer material in the gel fiber, the dynamic culture of cells can promote the functionalization of tissues and organs, and a large cell structure with a nutrient network can be obtained. The present invention is suitable for printing various complex tissue structures.

Description

technical field [0001] The invention relates to the technical field of biological manufacturing, in particular to a biological 3D printing method based on a nutrient flow channel. Background technique [0002] In recent years, with the development of 3D printing technology, it is gradually used in the field of tissue engineering and regenerative medicine. Our human body and most of our tissues need a lot of nutrients / oxygen, which is also true for tissue engineering structures. In fact, cells in the core of engineered tissue structures will die due to nutrient deprivation and hypoxia, so expanding the nutrient network within the cell-filled tissue structure is critical, promoting the formation of functional tissues, although tissue engineering has made great strides, but to date, creating large-scale organizational structures with nutritional network requirements remains a formidable challenge. [0003] In this context, three-dimensional (3D) bioprinting, an emerging techno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B29C64/106B29C64/379B29C64/40B33Y10/00B33Y40/00B33Y40/20B33Y70/10
CPCB29C64/106B29C64/379B29C64/40B33Y10/00B33Y40/00B33Y70/00
Inventor 贺永陈路路邵磊高庆
Owner 苏州永沁泉智能设备有限公司
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