A diabetic foot wound exudate detection chip

A technology for diabetic foot and exudate, which is applied in the direction of measuring devices, disease diagnosis, instruments, etc., to achieve the effect of ensuring accuracy

Active Publication Date: 2022-06-21
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of effective means to quickly detect the recovery of the diseased foot of diabetic foot patients in the existing technology. Therefore, it is urgent to find a detection product that can quickly detect the recovery of the diseased foot of diabetic foot patients.

Method used

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  • A diabetic foot wound exudate detection chip
  • A diabetic foot wound exudate detection chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Detection pad for diabetic foot wound exudate detection

[0039] A test pad for detecting diabetic foot wound exudate, such as figure 1 As shown, the material of the detection pad is nitrocellulose membrane, and the detection pad is provided with a sample adding area 1, a channel area 2, a reaction area 3 and a water absorption area 4 connected in sequence along the length direction of the detection pad.

[0040] The channel region 2 includes a non-accelerating channel 5, the inlet end of the non-accelerating channel 5 is communicated with the sample adding region 1, the outlet end of the non-accelerating channel 5 is communicated with the reaction zone 3, and the outlet end of the non-accelerating channel 5 is a constriction structure, The outlet end of the non-accelerating channel 5 is provided with a color developer coating area 8, and the color developer coating area 8 is coated with a color developer BCIP / NBT; both sides of the non-accelerating channel 5...

Embodiment 2

[0048] Example 2: Diabetic foot wound exudate detection chip

[0049] A diabetic foot wound exudate detection chip, such as figure 2 As shown, it includes a base 16, the base 16 is a PVC board, and the test pad for diabetic foot wound exudate detection prepared in Example 1 is pasted on the base 16, and the sample pad 15 is provided on the sample adding area 1 of the test pad, One end of the sample pad 15 is overlapped on the sample adding area 1 of the detection pad, and the other end of the sample pad 15 is pasted on the base 16; the water absorption area 4 of the detection pad is provided with an absorption pad 14, and one end of the absorption pad 14 is overlapped on the substrate 16. On the water absorption area 4 of the detection pad, the other end of the absorption pad 14 is pasted on the base 16 . The material of the sample pad 15 is glass cellulose film, and the material of the absorbent pad 14 is absorbent filter paper.

[0050] The specific method for detecting t...

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Abstract

The invention belongs to the technical field of microfluidic chips, and discloses a diabetic foot wound exudate detection chip. The detection chip includes a substrate, on which a detection pad is arranged, and a sample loading area, a channel area, a reaction area and a water absorption area are arranged on the detection pad; Connected with the reaction area, the non-accelerated channel is provided with a chromogen-coated area, and the non-accelerated channel is provided with an accelerated channel; a hydrophobic interval is provided between the non-accelerated channel and the accelerated channel; the entrance of the accelerated channel is connected to the sample loading area , the outlet end is provided with an oblique channel, the acceleration channel communicates with the reaction area through the oblique channel, and an antibody coating area is arranged on the acceleration channel; a detection line and a quality control line are arranged in the reaction area, and the outlet end of the reaction area is connected to the water absorption area. connected. The detection chip of the present invention can quickly detect whether MMP-9 is contained in the exudate of the wound of the diabetic foot patient, so as to know the recovery situation of the patient's wound in time, and provide help for follow-up treatment.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip detection, in particular to a diabetic foot wound exudate detection chip. Background technique [0002] Microfluidic chips have attracted much attention because they can integrate testing items in traditional laboratories on a tiny chip, and have been widely used in biomedical, environmental and food testing fields. [0003] Point of Care Test (POCT) is an important area of ​​in vitro diagnostics; it eliminates the need for patients to go to the hospital to undergo complex and time-consuming biochemical tests, and only needs a miniaturized device to get the desired results . At present, blood glucose meters are the most successful POCT devices; patients can check their blood sugar status anytime, anywhere, and no longer need to go to the hospital for testing. [0004] Microfluidic paper analysis equipment, as a branch of microfluidic chips, has many advantages compared to traditional a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/573
CPCG01N33/558G01N33/573G01N2333/96494G01N2800/042
Inventor 岳涛顾申瑜刘媛媛刘娜兰伟霞高守玮蔚延聪李恒宇谢少荣罗均
Owner SHANGHAI UNIV
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