Extraction preparation method of ganoderan for animals

A technology for Ganoderma lucidum polysaccharides and animal use, which is applied in the field of extraction and preparation of Ganoderma lucidum polysaccharides for animals. It can solve the problems of low extraction rate of Ganoderma lucidum polysaccharides, achieve the effects of improving immunity, increasing extraction rate, and broad market prospects

Inactive Publication Date: 2021-06-11
江西嘉博生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the present invention uses inferior ganoderma lucidum or ganoderma residues in the production and preparation process as extraction raw materials, recycles inferior ganoderma lucidum or ganoderma residues while reducing costs, and reduces the waste of ganoderma resources; at the same time, the extraction rate of the existing ganoderma polysaccharide extraction technology is low The present invention provides a method for extracting and preparing Ganoderma lucidum polysaccharides for animals: removing protein while reducing the loss of Ganoderma lucidum polysaccharides through ultrasound and changing the pH value in the ultrasonic process, so that the purity and extraction rate of Ganoderma lucidum polysaccharides are greatly improved, and the synergistic surface The increased solubilization of the active agent sorbitan monolaurosilicate also significantly increased the yield of polysaccharides from Ganoderma lucidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This embodiment provides a method for extracting and preparing Ganoderma lucidum polysaccharides for animals, including:

[0021] S1. Slice and pulverize inferior ganoderma lucidum or ganoderma lucidum residue in the production process, use ethyl acetate Soxhlet extraction to remove fat, then soak in normal saline, and filter to collect the precipitate;

[0022] S2. Add 1.2% sorbitan monolaurate dispersion to the precipitate obtained in S1 at a mass volume ratio of 1:20 g / mL, and soak for 5 hours to obtain a feed liquid;

[0023] S3. Adjusting the pH of the feed liquid obtained in S2 to 7.0, and then ultrasonically extracting (55° C., 350 W, extraction for 15 min) for 3 times, and then combining the extracts to obtain a Ganoderma lucidum polysaccharide extract;

[0024] S4, the Ganoderma lucidum polysaccharide extract is centrifuged (5000r / min, 8min), and the supernatant is washed with H 2 o 2 Decolorization, Sevage method to remove protein (in H 2 o 2 After decolor...

Embodiment 2

[0026] This embodiment provides a method for extracting and preparing Ganoderma lucidum polysaccharides for animals, including:

[0027] S1. Slice and pulverize inferior ganoderma lucidum or ganoderma lucidum residue in the production process, use ethyl acetate Soxhlet extraction to remove fat, then soak in normal saline, and filter to collect the precipitate;

[0028] S2. Add 1.0% sorbitan monolaurate dispersion to the precipitate obtained in S1 at a mass volume ratio of 1:15 g / mL, and soak for 4 hours to obtain a feed liquid;

[0029] S3. Adjusting the pH of the feed liquid obtained in S2 to 6.0, and then ultrasonically extracting (45° C., 280 W, extraction for 12 minutes) for 3 times, and then combining the extracts to obtain a Ganoderma lucidum polysaccharide extract;

[0030] S4, Ganoderma lucidum polysaccharide extract is centrifuged (4200r / min, 6min), and the supernatant is washed with H 2 o 2 Decolorization, Sevage method to remove protein (in H 2 o 2 After decolor...

Embodiment 3

[0032] This embodiment provides a method for extracting and preparing Ganoderma lucidum polysaccharides for animals, including:

[0033] S1. Slice and pulverize inferior ganoderma lucidum or ganoderma lucidum residue in the production process, use ethyl acetate Soxhlet extraction to remove fat, then soak in normal saline, and filter to collect the precipitate;

[0034] S2. Add 1.5% sorbitan monolaurate dispersion to the precipitate obtained in S1 at a mass volume ratio of 1:25 g / mL, and soak for 6 hours to obtain a feed liquid;

[0035] S3. Adjusting the pH of the feed liquid obtained in S2 to 8.0, and then ultrasonically extracting (60° C., 400 W, extraction for 20 min) 3 times, and then combining the extracts to obtain a Ganoderma lucidum polysaccharide extract;

[0036] S4, Ganoderma lucidum polysaccharide extract is centrifuged (5200r / min, 10min), gets the supernatant with H 2 o 2 Decolorization, Sevage method to remove protein (in H 2 o 2After decolorization, add 1 / 4 ...

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Abstract

The invention provides an extraction preparation method of ganoderan for animals. The method comprises the following steps: slicing and crushing ganoderma lucidum, performing Soxhlet extraction by using ethyl acetate to remove fat, performing filtering to obtain a precipitate, adding a surfactant sorbitan monolaurate for soaking, adjusting the pH value, performing ultrasonic extraction, performing centrifugal separation on an extracting solution, decoloring supernate with H2O2, removing proteins by a Sevage method, and performing concentrating, alcohol precipitation and freeze-drying to obtain the high-purity ganoderan. According to the invention, glycopeptide bonds of glycoprotein are broken through ultrasonic treatment and change of the pH value in the ultrasonic treatment process, in addition, the dissolution of the ganoderan is increased by adopting the surfactant sorbitan monolaurate, and the extraction rate of the ganoderan is remarkably increased. The synergistic effect of the two means increases the yield of the ganoderan. The method is simple and convenient to operate, takes less time, is nontoxic and pollution-free while efficiently extracting the ganoderan, can remarkably improve the immunity of the poultry and livestock and promote the growth of the poultry and livestock, can be widely applied to the poultry and livestock, and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of ganoderma polysaccharide extraction, and in particular relates to an extraction and preparation method of ganoderma polysaccharide for animals. Background technique [0002] Ganoderma lucidum belongs to Mycophyta, Basidiomycota, Laminomycetes, Ganoderma, and Ganodermaceae in systematic classification. Ganoderma lucidum is rich in polysaccharides, triterpenoids, nucleosides, amino acids, sterols, alkaloids and other active ingredients. It has a variety of physiological functions and is a rare high-level nutritional food. Ganoderma lucidum polysaccharide is one of the main active ingredients of Ganoderma lucidum, which has the functions of anti-tumor, anti-oxidative free radicals, anti-aging, improving immunity, promoting blood circulation and removing blood stasis. [0003] The main medicinal ingredient of Ganoderma lucidum is Ganoderma lucidium Polysaccharide, which has a variety of pharmacological activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周志曹远东曾二生潘烘李成龙
Owner 江西嘉博生物工程有限公司
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