Extraction preparation method of ganoderan for animals

Abstract

The invention provides an extraction preparation method of ganoderan for animals. The method comprises the following steps: slicing and crushing ganoderma lucidum, performing Soxhlet extraction by using ethyl acetate to remove fat, performing filtering to obtain a precipitate, adding a surfactant sorbitan monolaurate for soaking, adjusting the pH value, performing ultrasonic extraction, performing centrifugal separation on an extracting solution, decoloring supernate with H2O2, removing proteins by a Sevage method, and performing concentrating, alcohol precipitation and freeze-drying to obtain the high-purity ganoderan. According to the invention, glycopeptide bonds of glycoprotein are broken through ultrasonic treatment and change of the pH value in the ultrasonic treatment process, in addition, the dissolution of the ganoderan is increased by adopting the surfactant sorbitan monolaurate, and the extraction rate of the ganoderan is remarkably increased. The synergistic effect of the two means increases the yield of the ganoderan. The method is simple and convenient to operate, takes less time, is nontoxic and pollution-free while efficiently extracting the ganoderan, can remarkably improve the immunity of the poultry and livestock and promote the growth of the poultry and livestock, can be widely applied to the poultry and livestock, and has a wide market prospect.

Description

technical field

[0001] The invention belongs to the technical field of ganoderma polysaccharide extraction, and in particular relates to an extraction and preparation method of ganoderma polysaccharide for animals. Background technique

[0002] Ganoderma lucidum belongs to Mycophyta, Basidiomycota, Laminomycetes, Ganoderma, and Ganodermaceae in systematic classification. Ganoderma lucidum is rich in polysaccharides, triterpenoids, nucleosides, amino acids, sterols, alkaloids and other active ingredients. It has a variety of physiological functions and is a rare high-level nutritional food. Ganoderma lucidum polysaccharide is one of the main active ingredients of Ganoderma lucidum, which has the functions of anti-tumor, anti-oxidative free radicals, anti-aging, improving immunity, promoting blood circulation and removing blood stasis.

[0003] The main medicinal ingredient of Ganoderma lucidum is Ganoderma lucidium Polysaccharide, which has a variety of pharmacological activ...