Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus subtilis, culture method and application

A technology of Bacillus subtilis and its cultivation method, which is applied in the field of cultivation of Bacillus subtilis JNKC001, can solve the problems of residues and high cost, leaf yellowing, and yield decline, and achieve a remarkable effect of promoting production

Active Publication Date: 2021-06-11
金禾佳农(北京)生物技术有限公司
View PDF21 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the leaves of tangerines in some fields have been infected with anthracnose, which has caused serious leaf yellowing problems, and the quality and yield of tangerines have declined. For this problem, most of them are prevented and controlled through agricultural operations.
The prevention and control of shatangju disease is also limited to the use of pesticides and field management. After the use of pesticides, the residue problems and costs are relatively high. Some use beneficial microorganisms for prevention and control, which is also limited to the optimal storage of shatangju fruits after picking. , seldom use beneficial microorganisms to treat Shatangju fruit trees

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis, culture method and application
  • Bacillus subtilis, culture method and application
  • Bacillus subtilis, culture method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The source and screening of embodiment 1 strain Bacillus subtilis

[0032] (1) Separation and purification of rhizobacteria

[0033] The soil samples were collected from the rhizosphere soil of Shatangju in Wuyou Village, Shechong Township, Luoman Town, Luoman Town, Liujiang County, Liuzhou City, Guangxi Zhuang Autonomous Region, using the "three-point sampling method", and were separated according to the conventional plate dilution method of soil microorganisms. Weigh about 10g of the rhizosphere soil sample, put it into a glass container filled with 100mL of sterile water and vibrate for 30 minutes, so that the soil particles in it are evenly dispersed, and after standing for 5 minutes, take the supernatant part of the soil suspension to perform a 10-fold gradient Diluted sequentially to 10 -3 、10 -4 、10 -5 , take 0.1mL of the diluted solution and apply it to the beef extract peptone medium plate (peptone 7g, NaCl 5g, beef extract 3g, agar 20g, H 2 (0 1L, pH 7), a...

Embodiment 2

[0036] The cultivation method of embodiment 2 bacillus subtilis

[0037] (1) Take the activated Bacillus subtilis (Bacillus subtilis) JNKC001, inoculate it in the seed medium, and cultivate it for 10-12 hours at a temperature of 28-32°C and a rotation speed of 180-240r / min to obtain liquid seeds ;

[0038] (2) Take the liquid seeds prepared in step (1), inoculate them in the fermentation medium at a ratio of 2% by volume, and cultivate them for 28 to 40 hours at a temperature of 28 to 32° C. and a rotating speed of 180 to 240 r / min. , to prepare the fermentation broth.

[0039] Also include the step of activating the Bacillus subtilis (Bacillus subtilis) JNKC001 before step (1):

[0040] Get Bacillus subtilis (Bacillus subtilis) JNKC001, and the slant is transferred to the medium for activation;

[0041] Wherein, the activation temperature is 28-32° C., and the incubation time is 12-16 hours.

[0042] The components of the activation medium are as follows:

[0043] Pepton...

experiment example

[0050] Experimental example The fermentation liquid prepared by Bacillus subtilis (Bacillus subtilis) JNKC001 was applied to Shatang orange

[0051] There were 2 treatments in this experiment, and each treatment was repeated 3 times. 15 plants of Shatangju were randomly selected for each repetition, and a total of 6 plots were set up. Each plot was randomly arranged, with a total of 90 plants of Shatangju.

[0052] Treatment 1 (control group): spray 50mL / tree / time on the leaves of Shatangju with clean water, and spray once every 20 days.

[0053] Treatment 2 (experimental group): use Bacillus subtilis (Bacillus subtilis) JNKC001 bacterial solution diluted 20 times to spray the leaves of sugar orange, the dosage of Bacillus subtilis (Bacillus subtilis) JNKC001 bacterial solution is 50mL / tree / time, interval 20d Spray once.

[0054] 1. Chlorophyll content in the leaves of Shatangju

[0055] Five shatangju plants were randomly selected in each plot, and each plant was divided in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bacillus subtilis JNKC001 as well as a culture method and application thereof, and belongs to the technical field of microorganisms. The bacillus subtilis JNKC001 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is November 20, 2017, and the preservation number is CGMCC NO.14929. The bacillus subtilis JNKC001 provided by the invention has a good prevention effect on the citrus anthracnose; the growth and production promoting effects on the citrus are good; and the bacillus subtilis JNKC001 is simple in culture condition, short in culture period and easy to industrially produce and preserve, and has a good development and application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a cultivation method and application of Bacillus subtilis JNKC001. Background technique [0002] Citrus has a relatively large planting area in the south of my country and is one of the main fruits grown in the south. Among them, Shatangju is one of the dominant varieties of citrus. It is easy to peel, has a lot of juice, is seedless, and has a good taste. It has many functions such as clearing the intestines, strengthening the spleen, moistening the lungs, and reducing phlegm, so it is deeply loved by consumers. Not only sold in all provinces and cities of the country, but also exported to all over the world, the market is broad. [0003] Anthracnose is the most common disease of citrus. It has the characteristics of wide damage and long-term damage. It mainly harms leaves, branches, flowers, fruits and fruit stalks. It often causes a large number of citrus varieties suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCC12N1/20A01N63/22Y02P60/87
Inventor 张丽霞
Owner 金禾佳农(北京)生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com