Recovery processing method of traditional Chinese medicine waste residue, phellinus igniarius residue fermentation compound and application
A technology for the recycling and treatment of waste residues of traditional Chinese medicine, which is applied in the fields of application, drug combination, and medical formula. It can solve the problems of waste of traditional Chinese medicine resources, low utilization efficiency of Chinese medicinal materials, and environmental pollution, and achieve recycling, easy animal digestion, and beneficial development. The effect of growth
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Embodiment 1
[0036] (1) Preparation of medicinal dregs culture substrate: dry and pulverize 80 mass parts of compound Ganmaoling tablet medicinal dregs (honeysuckle, five-finger mandarin orange, wild chrysanthemum, Sanchaku, Nanbanlangen, Gangmei), add 18 mass parts of wheat bran, 1 mass part of gypsum powder and 1 mass part of quicklime are mixed evenly, soaked with water to make the water content of the medicinal residue culture substrate 50%, then bagged with polyethylene plastic bags (1kg / bag), and sterilized at 1.4MP for 2.5h to obtain Medicinal residue culture substrate;
[0037] (2) Inoculate the mother species of Aspergillus niger on the potato dextrose agar medium for activation culture, cultivate it for 3 days in a constant temperature incubator at 28°C, and then elute the activated Aspergillus niger with an appropriate amount of sterile ultrapure water, Make a spore suspension, transfer to 100mL liquid potato medium, adjust the concentration to 1×10 7 Individual / mL, in a consta...
Embodiment 2
[0041] (1) Preparation of medicinal dregs culture substrate: dry and pulverize 85 parts by mass of compound Ganmaoling tablet medicinal dregs (honeysuckle, five-finger orange, wild chrysanthemum, three-fork bitter, Nanbanlangen, Gangmei), add 15 parts by mass of wheat bran, 2 parts by mass of gypsum powder and 2 parts by mass of quicklime are mixed evenly, soaked with water to make the water content of the medicinal dregs culture substrate 60%, then bagged with a polyethylene plastic bag (1.5kg / bag), and 1.4MP is kept for 3h to sterilize to obtain the medicine. slag culture substrate;
[0042] (2) Inoculate the mother species of Aspergillus niger on the potato dextrose agar medium for activation culture, cultivate it in a constant temperature incubator at 32°C for 2 days, then elute the activated Aspergillus niger with an appropriate amount of sterile ultrapure water, Make a spore suspension, transfer to 100mL liquid potato medium, adjust the concentration to 1×10 7 cells / mL,...
Embodiment 3
[0046] (1) Preparation of medicinal dregs culture substrate: dry and pulverize 82 mass parts of compound Ganmaoling tablet medicinal dregs (honeysuckle, five-finger mandarin orange, wild chrysanthemum, Sanchaku, Nanbanlangen, Gangmei), add 20 mass parts of wheat bran, 1 part by mass of gypsum powder and 2 parts by mass of quicklime are mixed evenly, soaked with water to make the water content of the medicinal dregs culture substrate 55%, then bagged with a polyethylene plastic bag (1.2kg / bag), kept at 100°C for 12h to sterilize to obtain the medicinal product. slag culture substrate;
[0047] (2) Inoculate the parent species of Aspergillus niger on the potato dextrose agar medium for activation culture, cultivate it in a constant temperature incubator at 30°C for 2.5 days, and then elute the activated Aspergillus niger with an appropriate amount of sterile ultrapure water , made spore suspension, transferred to 100mL liquid potato medium, the concentration was adjusted to 1×10...
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