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Self-assembled multi-layer animal-derived protein hydrogel and preparation method and use thereof

An animal-derived protein, multi-layer technology, applied in the field of protein hydrogels, can solve the problems of delivery of active ingredients, less research on lipid substances, etc.

Inactive Publication Date: 2021-06-18
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the self-assembly of myosin in muscle mainly focuses on the influence of ionic strength and pH on its morphology, surface charge and structure, while there are few studies on the delivery of active ingredients and lipid substances.

Method used

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  • Self-assembled multi-layer animal-derived protein hydrogel and preparation method and use thereof
  • Self-assembled multi-layer animal-derived protein hydrogel and preparation method and use thereof
  • Self-assembled multi-layer animal-derived protein hydrogel and preparation method and use thereof

Examples

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preparation example Construction

[0030] The invention provides a method for preparing a self-assembled multi-layered animal-derived protein hydrogel, comprising the following steps:

[0031] Dispersing myosin in phosphate buffer to obtain a myosin dispersion, adjusting the pH to 5.0-9.0, and heating to obtain a self-assembled multi-layered animal-derived protein hydrogel;

[0032] The heating temperature is 75-95° C., and the heating time is 30-45 minutes.

[0033] In the present invention, unless otherwise specified, the required preparation materials are commercially available products well known to those skilled in the art.

[0034] The invention disperses the myosin in the phosphate buffer solution to obtain the myosin dispersion. In the present invention, the myosin is preferably derived from pigs; the present invention has no special limitation on the extraction method of the myosin, and extraction and purification are carried out according to a process well known in the art, which can make the myosin ...

Embodiment 1

[0045] Sequentially extracting and purifying the longissimus dorsi of pigs to obtain myosin (purity 90%);

[0046] Disperse 0.2 g of myosin in phosphate buffer (20 mL, 20 mm) containing NaCl (0.6 M, pH 7.0), and stir the above myosin dispersion (10 mg / mL) at 10 °C until fully hydrated Finally, the pH value was adjusted to 5.0 with hydrochloric acid aqueous solution (concentration 1mol / L), then heated in a water bath at 75°C for 30 minutes, and cooled to room temperature to obtain self-assembled multi-layered animal-derived protein hydrogels (ie, aggregates);

[0047] The self-assembled multi-layered animal-derived protein hydrogel prepared in Example 1 at a concentration of 0.6MNaCl was tested for potential, particle size, and protein secondary structure, and the particle size and particle size of the hydrogel were measured by a BT-9300ST laser particle size distribution analyzer. For particle size distribution, the detection parameters are set as follows: the medium is set to...

Embodiment 2

[0052] The difference between this embodiment and embodiment 1 is only: no need to adjust the pH value, that is, the pH value is 7.0, and the others are the same as embodiment 1.

[0053] According to the method of Example 1, the self-assembled multi-layered animal-derived protein hydrogel prepared under the concentration of 0.6M NaCl was tested, and the results showed that when the pH value was 7.0, the potential of the myosin hydrogel (0.6M NaCl) was -5.00mV, the average particle size is 540.97nm, the α-helix content is 31.51%, and the β-sheet is 37.63%.

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Abstract

The invention provides self-assembled multi-layer animal-derived protein hydrogel and a preparation method and use thereof, and belongs to the technical field of protein hydrogel. By controlling a pH value and changing an electrostatic repulsive force, myosin is aggregated at different degrees to form a myosin aggregate, the myosin aggregate is self-assembled in a heating process, a form, a particle size and a potential of the aggregate are all changed, a secondary structure of the protein is changed, and the multi-layer (two-dimensional and three-dimensional network structure) hydrogel is formed. The hydrogel has a realtively small average particle size (a nanometer level) and high stability. The hydrogel has high alpha-helix content, low beta-pleated sheet content and low alpha-helix-to-beta-pleated sheet conversion degree. Under the secondary structure, the hydrogel forms a certain regularly arranged characteristic form, the system can form a stable emulsified gel system, and the viscoelastic property of the system is improved, such that the hydrogel can well adsorb grease and deliver active ingredients, and has a widened use field in food.

Description

technical field [0001] The invention relates to the technical field of protein hydrogels, in particular to a self-assembled multi-layered animal-source protein hydrogel and its preparation method and application. Background technique [0002] Natural protein hydrogels are non-toxic, degradable, and have a three-dimensional network structure. They can be used as functional materials such as bioactive component carriers and protectors, so they have strong attraction and can be prepared by electrostatic interaction and thermal gelation. . Myosin is a major meat protein that plays an important role in water retention, emulsification and gelling properties. Myosin has a Y-shaped characteristic of a double-headed structure with a flexible neck region containing myosin light chains and a longer coiled tail domain, consisting of two heavy chains of about 220 kDa and four light chains of about 16-25 kDa composition. In emulsified meat products, myosin undergoes gelation to form a ...

Claims

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Application Information

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IPC IPC(8): A23L29/281A23L29/00
CPCA23L29/281A23L29/015A23V2002/00
Inventor 邵俊花韩宗元高小溪宋阳李春强岳喜庆孙京新王道营
Owner SHENYANG AGRI UNIV
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