Anti-glycation collagen peptide and preparation method thereof

A collagen peptide and collagen technology, applied in the field of anti-glycation collagen peptide and its preparation, can solve the problem of no anti-glycation collagen peptide, etc., and achieve the effects of improving blood vessel elasticity, being easy to digest, and improving skin moisture.

Active Publication Date: 2021-06-25
BEIJING SEMNL BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to research reports, collagen peptides have the effects of improving skin moisture, reducing wrinkles, and anti-aging, but there is no report on anti-glycation collagen peptides

Method used

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  • Anti-glycation collagen peptide and preparation method thereof
  • Anti-glycation collagen peptide and preparation method thereof
  • Anti-glycation collagen peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An anti-glycation collagen peptide made by the following steps:

[0026] Step S1 Preparation of the total protein crude extract: Weigh the raw material, add sodium hydroxide solution to soak for 2 hours, wash twice, add demineralized water, and homogenize in a grinder to a paste to obtain the total protein crude extract;

[0027] Step S2 Preparation of the crude collagen extract: adjust the pH value of the crude total protein extract to 2, heat to 75° C., and keep the gel for 3 hours to obtain the crude collagen extract;

[0028] Step S3 refining of the crude collagen extract: adding a clarifier to the crude collagen extract, adjusting the pH value to 2, stirring evenly and then standing for 2 hours to obtain a refined collagen product;

[0029] Step S4 preparation of collagen peptide: adjust the pH value of the refined collagen to 5, add complex protease for enzymatic hydrolysis, and enzymatically hydrolyze for 2 hours at a temperature of 45° C. to obtain an enzymatic ...

Embodiment 2

[0032] An anti-glycation collagen peptide made by the following steps:

[0033] Step S1 Preparation of the total protein crude extract: Weigh the raw material and add sodium hydroxide solution to soak for 3 hours, wash 3 times, add demineralized water, and homogenize in a grinder to a paste to obtain the total protein crude extract;

[0034] Step S2 Preparation of the crude collagen extract: adjust the pH value of the crude total protein extract to 3, heat to 80° C., and keep the gel for 5 hours to obtain the crude collagen extract;

[0035] Step S3 refining of the crude collagen extract: adding a clarifying agent to the crude collagen extract, adjusting the pH value to 5, stirring evenly and then standing for 5 hours to obtain a refined collagen product;

[0036] Step S4 preparation of collagen peptide: adjust the pH value of the refined collagen to 7, add complex protease for enzymatic hydrolysis, and enzymatically hydrolyze for 4 hours at a temperature of 50° C. to obtain a...

Embodiment 3

[0039] An anti-glycation collagen peptide made by the following steps:

[0040] Step S1 Preparation of the total protein crude extract: Weigh the raw material, add sodium hydroxide solution to soak for 4 hours, wash 3 times, add demineralized water, homogenize in a grinder to a paste, and obtain the total protein crude extract;

[0041] Step S2 Preparation of the crude collagen extract: adjust the pH value of the crude total protein extract to 4, heat to 90° C., and keep the gel for 8 hours to obtain the crude collagen extract;

[0042] Step S3 refining of the crude collagen extract: adding a clarifier to the crude collagen extract, adjusting the pH value to 7, stirring evenly and then standing for 8 hours to obtain a refined collagen product;

[0043] Step S4 preparation of collagen peptide: adjust the pH value of the refined collagen to 8, add complex protease for enzymatic hydrolysis, and enzymolyze for 6 hours at a temperature of 60° C. to obtain an enzymatic hydrolysis so...

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Abstract

The invention discloses an anti-glycation collagen peptide and a preparation method thereof. The anti-glycation collagen peptide is prepared by the following steps: S1, preparing a total protein crude extract; S2, preparing a crude collagen extract; S3, refining the crude collagen extract; S4, preparing the collagen peptide; S5, preparing the anti-glycation collagen peptide; The prepared anti-glycation collagen peptide is rich in G 1 y-Hyp-Pro-G 1 y-Arg-Pro-G 1 y-Hyp sequences, the molecular weight range is 850-1800 Daltons, and the anti-glycation collagen peptide is safe, is free of toxic and side effects, is easy to digest, exhibits an anti-glycation effect, can be used as a raw material of drugs, health-care foods and foods, can be added into acid beverages, and can be used for improving skin moisture wrinkles and improving vascular elasticity.

Description

technical field [0001] The invention relates to the technical field of polypeptide synthesis, in particular to an anti-glycation collagen peptide and a preparation method thereof. Background technique [0002] In the 20th century, the average human life expectancy increased by 30 years, and aging has aroused great interest of researchers. Aging is a complex process involving multiple mechanisms, including accumulation of genomic mutations, accumulation of toxic metabolites, hormone reduction, and macromolecular cross-linking due to glycation. Glycation reaction is a non-enzymatic reaction between free amino groups and reducing sugars, that is, the glycosylation reaction of proteins. This reaction can produce glycation end products (advancedglycationendproducts, AGE), such as carboxymethyllysine, carboxyethyllysine, glycosylated collagen and so on. Since the collagen in the extracellular matrix of the skin tissue has low reversibility, it is more likely to undergo non-enzym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/18C07K1/16C07K1/14C07K1/36
CPCC07K14/78C12P21/06Y02P20/10
Inventor 王海燕刘爱青闫征
Owner BEIJING SEMNL BIOTECHNOLOGY CO LTD
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