Perfusion type reactor for in-vitro dynamic culture of cells

Abstract

The invention discloses a perfusion reactor for in-vitro dynamic culture of cells, and belongs to the technical field of cell dynamic culture devices. The perfusion reactor comprises a peristaltic pump, a perfusion chamber and a liquid storage bottle, the peristaltic pump, the perfusion chamber and the liquid storage bottle are connected through conduits, and a scaffold is arranged in the perfusion chamber. The perfusion type reactor for in-vitro dynamic culture of cells is simple in composition structure and easy to build, in the cell culture process, the hydrogel scaffold is placed in the perfusion chamber, a culture solution is placed in the liquid storage bottle, the peristaltic pump is opened, the flow rate is adjusted, the culture solution circularly flows to culture cells, the shearing force applied to the interior of the scaffold is obtained through finite element analysis, and the magnitude of the shearing force applied to the scaffold can be adjusted by adjusting the flow velocity, so that the influence of too large or too small shearing force on the proliferation effect of cartilage cells is avoided.

Description

technical field [0001] The invention relates to the technical field of cell dynamic culture devices, and more specifically relates to a perfusion reactor for dynamic culture of cells in vitro. Background technique [0002] The damaged articular cartilage is difficult to self-repair or regenerate, and the development of tissue engineering provides the possibility for the repair of articular cartilage. The reason for the extremely poor self-repair ability of articular cartilage tissue after injury is that there are no nerves and blood vessels in the tissue, and different cultures The way will lead to different morphology of chondrocytes, and its biological characteristics are also very different. [0003] At present, the culture methods of cells in vitro are divided into static culture and dynamic culture. Static culture is generally cultured directly from a petri dish. Dynamic culture is divided into three-dimensional hydrogel scaffold culture, various types of bioreactor a...

AI Technical Summary

Problems solved by technology

The defect is that the self-made perfusion bioreactor cannot control the shear force on the inside of the scaffold. If the shear force is too large, the hydrogel scaffold will break, and if the shear force is too small, the proliferation of chondrocytes in the perfusion bioreactor will not be achieved. to the expected effect

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