Perfusion type reactor for in-vitro dynamic culture of cells

A dynamic culture and perfusion technology, applied in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, bioreactor/fermenter combinations, etc. The chondrocyte proliferation effect of the reactor is not as expected, and the shear force of the scaffold cannot be controlled, so as to achieve the effect of easy construction and simple composition

Pending Publication Date: 2021-06-25
HANGZHOU DIANZI UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect is that the self-made perfusion bioreactor cannot control the shear force on the inside of the scaffold. If the shear force is too large, the hydrogel scaffold will break, and if the shear force is too small, the proliferation of chondrocytes in the perfusion bioreactor will not be achieved. to the expected effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Perfusion type reactor for in-vitro dynamic culture of cells
  • Perfusion type reactor for in-vitro dynamic culture of cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0032] refer to Figure 1-Figure 2 , a perfusion reactor for dynamic culture of cells in vitro, comprising a peristaltic pump 1, a perfusion chamber 2 and a liquid storage bottle 3, the peristaltic pump 1, the perfusion chamber 2 and the liquid storage bottle 3 are connected by a conduit 4, and the perfusion chamber 2 is set There are brackets.

[0033] More specifically, the scaffold is a hydrogel scaffold.

[0034] A method for improving the effect of cell proliferation with a perfusion reactor for dynamic culture, comprising the following steps:

[0035] Step 1. Calculate the flow rate inside the perfusion reactor. The theoretical calculation formula of the flow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a perfusion reactor for in-vitro dynamic culture of cells, and belongs to the technical field of cell dynamic culture devices. The perfusion reactor comprises a peristaltic pump, a perfusion chamber and a liquid storage bottle, the peristaltic pump, the perfusion chamber and the liquid storage bottle are connected through conduits, and a scaffold is arranged in the perfusion chamber. The perfusion type reactor for in-vitro dynamic culture of cells is simple in composition structure and easy to build, in the cell culture process, the hydrogel scaffold is placed in the perfusion chamber, a culture solution is placed in the liquid storage bottle, the peristaltic pump is opened, the flow rate is adjusted, the culture solution circularly flows to culture cells, the shearing force applied to the interior of the scaffold is obtained through finite element analysis, and the magnitude of the shearing force applied to the scaffold can be adjusted by adjusting the flow velocity, so that the influence of too large or too small shearing force on the proliferation effect of cartilage cells is avoided.

Description

technical field [0001] The invention relates to the technical field of cell dynamic culture devices, and more specifically relates to a perfusion reactor for dynamic culture of cells in vitro. Background technique [0002] The damaged articular cartilage is difficult to self-repair or regenerate, and the development of tissue engineering provides the possibility for the repair of articular cartilage. The reason for the extremely poor self-repair ability of articular cartilage tissue after injury is that there are no nerves and blood vessels in the tissue, and different cultures The way will lead to different morphology of chondrocytes, and its biological characteristics are also very different. [0003] At present, the culture methods of cells in vitro are divided into static culture and dynamic culture. Static culture is generally cultured directly from a petri dish. Dynamic culture is divided into three-dimensional hydrogel scaffold culture, various types of bioreactor a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12M1/24
CPCC12M23/08C12M23/20C12M25/14C12M29/10C12M29/18C12M35/04
Inventor 华燕史廷春
Owner HANGZHOU DIANZI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap