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Fluid infusion culture medium and method for culturing and inducing hematopoietic stem cells to differentiate into megakaryocyte cells
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A technology of hematopoietic stem cells and medium, applied in the field of bioengineering, can solve the problems of low induction differentiation efficiency and weak proliferation ability
Pending Publication Date: 2021-06-25
ACADEMY OF MILITARY MEDICAL SCI +1
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[0003] However, the current system for promoting the induction and differentiation of hematopoietic stem cells into megakaryocytes still has problems such as weak proliferation ability and low induction differentiation efficiency.
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Embodiment 1
[0071] Materials and methods
[0072] 1. Main materials
[0073] stemspan TM II serum-free medium is the product of stemcell company;
[0074] Recombinant human thrombopoietin injection is Shenyang Sansheng Pharmaceutical Co., Ltd.;
[0075] SCF, IL-3, and IL-6 are all products of RD;
[0105] Aspartic acid L-ASparagine (Asp): 1M HCl configuration, mother solution concentration 757mM, culture system final concentration 124μM;
[0106] L-cysteine (Cyss): H 2 O configure the mother solution concentration of 206.3mM, and the final concentration of the culture system is 44μM;
[0107] Serine L-serine (Ser): H 2 O is used to configure the concentration of the mother solution to be 475.78mM, and the final concentration of the culture system to be 350μM.
[0108] 2. Induction of differentiation of umbilical cord blood mononuclear cells into megakaryocytes
[0109] The isolated umbilical cord blood mononuclear cells were divided into 5×10 5 piece / cm 2 Inoc...
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technical field [0001] The invention relates to the field of bioengineering. Specifically, the present invention relates to a rehydration medium and a method for inducing hematopoietic stem cells to differentiate into megakaryocytes. Background technique [0002] Platelets are produced by the cytoplasm of mature megakaryocytes in the bone marrow and have the functions of coagulation and capillary protection. Abnormal platelets can lead to bleeding disorders. In recent years, the incidence of tumors has been increasing year by year. Radiotherapy and chemotherapy are commonly used methods for tumor treatment, often accompanied by symptoms of thrombocytopenia. Clinically, platelet transfusion is used as the main treatment, but blood supply is tight, or repeated platelet transfusions are ineffective. Prompting people to explore new sources of platelets. Hematopoietic stem cells can differentiate into megakaryotic progenitor cells in vitro, and gradually mature into platelets, ...
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